Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013622_eol7 GTGTCTACTTCGTAAGAGCCGTGTCTATCCAG(+ATATAT)GTACCAAAAGTGAGAGGTCAGCAGTGGGTGG NGG 10 8 17 8 24 0 100 5 10
    240607013621_tmrg CCAGCCCACCCACTGCTGACCTCTCACTTTTG(+ATATAT)GTACCTGGATAGACACGGCTCTTACGAAGTA NGG 10 8 17 8 24 0 100 5 10
    240607013619_anax CTGAGGACATCACGGAAGCCCAGAGATTAGGG(+ATATAT)GTACCACTGGAGGAGCCTCACTGCAGGAGCC NGG 10 8 17 8 24 0 100 5 10
    240607013617_qh1i CTCCAGGCTCCTGCAGTGAGGCTCCTCCAGTG(+ATATAT)GTACCCCTAATCTCTGGGCTTCCGTGATGTC NGG 10 8 17 8 24 0 100 5 10
    240607013616_l778 ATTGAAGCAGGGATGGCTTGTCATAGCAGTGG(+ATATAT)GATCCAGCTTCACCTGAGTTACTCTACCAAA NGG 10 8 17 8 24 0 100 5 10
    240607013615_zmq0 CTGTGTTTGGTAGAGTAACTCAGGTGAAGCTG(+ATATAT)GATCCCACTGCTATGACAAGCCATCCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240607013613_xvmz GACAGGAGCGCAGAGGTGAGAGAAAACAACAG(+ATATAT)GATCCTCCCAAGAAGTCCTTTCCTTAGGCCT NGG 10 8 17 8 24 0 100 5 10
    240607013612_fg3i CACAAAGGCCTAAGGAAAGGACTTCTTGGGAG(+ATATAT)GATCCTGTTGTTTTCTCTCACCTCTGCGCTC NGG 10 8 17 8 24 0 100 5 10
    240607013611_t083 GAGAATCCTGTTAAAATTGTTGGCTGTTACTG(+ATATAT)GTACCCTATGTTAATCAGCCACCAGGTGTCA NGG 10 8 17 8 24 0 100 5 10
    240607013609_96ps TGAAATGACACCTGGTGGCTGATTAACATAGG(+ATATAT)GTACCAGTAACAGCCAACAATTTTAACAGGA NGG 10 8 17 8 24 0 100 5 10