Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607012428_95ph GATCTTCTAGGCTTATTCTGTTCATTGGTGTG(+ATATAT)GATCCTGAAATTTTCACATCACAGCGGGGTT NGG 10 8 17 8 24 0 100 5 10
    240607012425_siis ACTGTCATATTTATACATGGCTTTTCCCCTAG(+ATATAT)GATCCTCAATCCTTCAGACCTAAACACAATT NGG 10 8 17 8 24 0 100 5 10
    240607012424_u14b CCTGAAATTGTGTTTAGGTCTGAAGGATTGAG(+ATATAT)GATCCTAGGGGAAAAGCCATGTATAAATATG NGG 10 8 17 8 24 0 100 5 10
    240607012422_bj3j GGCCCCAGGTGTGCCAGCAGCTCAGGACATGG(+ATATAT)GATCCAGTGGGCTCTCCTTCCTCTGCAGGTT NGG 10 8 17 8 24 0 100 5 10
    240607012421_x96v TGGAGAACCTGCAGAGGAAGGAGAGCCCACTG(+ATATAT)GATCCCATGTCCTGAGCTGCTGGCACACCTG NGG 10 8 17 8 24 0 100 5 10
    240607012419_c4lo CTCTATTCCATACTATATCCGTTGTGTGGCTG(+ATATAT)GTACCTTAGTGGGGAAGGATGTCTCAGCATG NGG 10 8 17 8 24 0 100 5 10
    240607012418_een0 GGGCCCATGCTGAGACATCCTTCCCCACTAAG(+ATATAT)GTACCAGCCACACAACGGATATAGTATGGAA NGG 10 8 17 8 24 0 100 5 10
    240607012417_tctl TTTTTTTCTAGCATGCTTAGGACCCTGAGTTG(+ATATAT)GATCCCCAGCACCAGAGGGCAACAGGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240607012415_lqtb TCTCTCTCTCCCTGTTGCCCTCTGGTGCTGGG(+ATATAT)GATCCAACTCAGGGTCCTAAGCATGCTAGAA NGG 10 8 17 8 24 0 100 5 10
    240607012413_2xge AAACAAAAGAGAATGAGAGCAAAGCATGATGG(+ATATAT)GTACCAAGCAACAAGCAAAGATCAAAATGAA NGG 10 8 17 8 24 0 100 5 10