Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607012355_3rwx CTCCAGATCTAGGGTTCTCCCTGTGCTTAGAG(+ATATAT)GATCCTCAGTGACAGTAACGTTCTCCAGTGG NGG 10 8 17 8 24 0 100 5 10
    240607012354_8fa2 CAGCCTGCCCTGTCTGTCACTGAGGGCTCTGG(+ATATAT)GATCCTAGTGGAGACAGGGACCGCTTAGAAG NGG 10 8 17 8 24 0 100 5 10
    240607012353_9l69 GGTCCCTTCTAAGCGGTCCCTGTCTCCACTAG(+ATATAT)GATCCCAGAGCCCTCAGTGACAGACAGGGCA NGG 10 8 17 8 24 0 100 5 10
    240607012351_rbfq TGTTTCTAAGAATTCATTCCATGAAAAATGTG(+ATATAT)GATCCTCTTTATACCACAGCATTTATACTAT NGG 10 8 17 8 24 0 100 5 10
    240607012350_09z1 GGAACATAGTATAAATGCTGTGGTATAAAGAG(+ATATAT)GATCCACATTTTTCATGGAATGAATTCTTAG NGG 10 8 17 8 24 0 100 5 10
    240607012348_1u45 TTCTGAAAAGGAAAAAAAAAAAAGCCTCCTTG(+ATATAT)GTACCCACACCTGGCTTCTCCACAATCCAGC NGG 10 8 17 8 24 0 100 5 10
    240607012347_kpb0 TGCCAGCTGGATTGTGGAGAAGCCAGGTGTGG(+ATATAT)GTACCAAGGAGGCTTTTTTTTTTTTCCTTTT NGG 10 8 17 8 24 0 100 5 10
    240607012346_v5re TTACCACCGAGGTCACTTCACTGAGGTAACTG(+ATATAT)GTACCTTAGTCTCTTGTGGCCACTTACAGAT NGG 10 8 17 8 24 0 100 5 10
    240607012344_i74i CCGATATCTGTAAGTGGCCACAAGAGACTAAG(+ATATAT)GTACCAGTTACCTCAGTGAAGTGACCTCGGT NGG 10 8 17 8 24 0 100 5 10
    240607012343_9ucs GCATTCCTCTTGCCCAGCCATGTTCACGGTTG(+ATATAT)GATCCTTAATGCATTGTGAGTGTTCCATCAA NGG 10 8 17 8 24 0 100 5 10