Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607012246_2do0 GGTATCTCTATCCTGGAGGCCACAGCTCCAGG(+ATATAT)GTACCTGTGGACAATGAATAAAGGCCCTCCG NGG 10 8 17 8 24 0 100 5 10
    240607012244_2ufx ATTCACCTGCTCTTTGGGCAAACATTCATTGG(+ATATAT)GTACCACAACACCAAACCTCCCGAGACAGGC NGG 10 8 17 8 24 0 100 5 10
    240607012243_3fc4 TAGTTGCCTGTCTCGGGAGGTTTGGTGTTGTG(+ATATAT)GTACCCAATGAATGTTTGCCCAAAGAGCAGG NGG 10 8 17 8 24 0 100 5 10
    240607012242_jz8n ATATCGGCTGAATACCGGCTGAACTAAAACTG(+ATATAT)GTACCCGCTGCCTTCAGTCCAGTGGGTCACA NGG 10 8 17 8 24 0 100 5 10
    240607012240_ciw1 CTGCCTGTGACCCACTGGACTGAAGGCAGCGG(+ATATAT)GTACCAGTTTTAGTTCAGCCGGTATTCAGCC NGG 10 8 17 8 24 0 100 5 10
    240607012239_4z2y AAAGACTCCCACCCCTGGCAGGAAGCAGGAAG(+ATATAT)GATCCGCCAGGCCTGGAGCATTCCTTCCTCC NGG 10 8 17 8 24 0 100 5 10
    240607012237_gau9 ACTGTGGAGGAAGGAATGCTCCAGGCCTGGCG(+ATATAT)GATCCTTCCTGCTTCCTGCCAGGGGTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240607012234_xb01 AACAATATAAAAAAGTACTCCCATAGTCCCAG(+ATATAT)GTACCTCCATCAAAAAAAAAAAAGAACTGGG NGG 10 8 17 8 24 0 100 5 10
    240607012233_4kpb TTGTCCCCAGTTCTTTTTTTTTTTTGATGGAG(+ATATAT)GTACCTGGGACTATGGGAGTACTTTTTTATA NGG 10 8 17 8 24 0 100 5 10
    240607012230_wk97 GGGAAAATCTAAAGGAGACCCCACGTGCCCAG(+ATATAT)GATCCGGGAACCAACCTTCCACACCACTCCA NGG 10 8 17 8 24 0 100 5 10