Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607012138_40lj GGGGGGGAAAGGAGTGTGGTGGGGTCCTGTGG(+ATATAT)GATCCAAAAAGGATTTAGACTGGAGATTTGG NGG 10 8 17 8 24 0 100 5 10
    240607012137_5n5y TGCTGAGACATGCTGGGCATGGCAGCTGTGAG(+ATATAT)GTACCTCCCACATCTGCTTTCTGGCTGTGCT NGG 10 8 17 8 24 0 100 5 10
    240607012135_lbho AAGTCAGCACAGCCAGAAAGCAGATGTGGGAG(+ATATAT)GTACCTCACAGCTGCCATGCCCAGCATGTCT NGG 10 8 17 8 24 0 100 5 10
    240607012133_2kcq CTCTTGTAGAAGCCAGTCAGGAACTGAGCCTG(+ATATAT)GATCCTGTCCACATAAGCCTGCCCAGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240607012132_jf0z TAGTGCTGCTCTGGGCAGGCTTATGTGGACAG(+ATATAT)GATCCAGGCTCAGTTCCTGACTGGCTTCTAC NGG 10 8 17 8 24 0 100 5 10
    240607012130_3z4o GCGCCCCCTGGCGGGACCCTTGACCCCCAAAG(+ATATAT)GATCCACCAGAGCAGTTGCTGTGCTCTCTAG NGG 10 8 17 8 24 0 100 5 10
    240607012129_x62d ATATTCTAGAGAGCACAGCAACTGCTCTGGTG(+ATATAT)GATCCTTTGGGGGTCAAGGGTCCCGCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240607012126_4aem GCTCCATTATTCCCCACACAAGACTCCCCTGG(+ATATAT)GTACCACGGAGAACTCCTCTAAAACCTCTTA NGG 10 8 17 8 24 0 100 5 10
    240607012124_slo2 GCACCTAAGAGGTTTTAGAGGAGTTCTCCGTG(+ATATAT)GTACCCAGGGGAGTCTTGTGTGGGGAATAAT NGG 10 8 17 8 24 0 100 5 10
    240607012122_c7l9 GCCGCCTGGAAGCGCTCAGTGCCACGGCTGGG(+ATATAT)GATCCTAGATCCTGCTGCATGGACCCTATAA NGG 10 8 17 8 24 0 100 5 10