Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607012212_ihs7 CTGATCTTCCCGCCTCCACCTCTACCTCCCAG(+ATATAT)GTACCAGGCCTTCAGGTATATTCAGCAAACC NGG 10 8 17 8 24 0 100 5 10
    240607012210_vyrf ACCACATAGTGGCTCACAACCATCTGCAATGG(+ATATAT)GATCCGATGCTCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240607012209_8xk3 GCTGTCTTCAGACACACCAGAAGAGAGCATCG(+ATATAT)GATCCCATTGCAGATGGTTGTGAGCCACTAT NGG 10 8 17 8 24 0 100 5 10
    240607012208_2p62 GCTTGTTTTCTCTTTGTAAAGGACAAACTATG(+ATATAT)GTACCAGAATCTGCCAAAGATCCCCTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240607012206_keah TGAGATGAGAAGGGGATCTTTGGCAGATTCTG(+ATATAT)GTACCATAGTTTGTCCTTTACAAAGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240607012203_2g62 CTTGCTTAGAGCCAGAAGGGATGATGTCCTGG(+ATATAT)GATCCAAGACCAGAGGGACAGCATCACTGAG NGG 10 8 17 8 24 0 100 5 10
    240607012202_l8g5 CTCCCCTCAGTGATGCTGTCCCTCTGGTCTTG(+ATATAT)GATCCCAGGACATCATCCCTTCTGGCTCTAA NGG 10 8 17 8 24 0 100 5 10
    240607012200_mshj GTACATGAGTGTGGAAGTCTGAGCTATATTTG(+ATATAT)GTACCCGATAGGATACTTTAGGTATCAGGAT NGG 10 8 17 8 24 0 100 5 10
    240607012159_eq0d ACTGAATCCTGATACCTAAAGTATCCTATCGG(+ATATAT)GTACCAAATATAGCTCAGACTTCCACACTCA NGG 10 8 17 8 24 0 100 5 10
    240607012157_18u3 CCCCAGGAAGGCAGTACCCTGCCCCTGGGAGG(+ATATAT)GTACCACTCACATGGAACCAGAGGAAGCTGC NGG 10 8 17 8 24 0 100 5 10