Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607012107_0adq CAGGCGGCTGCAGAGAAGACAGCTTGGGCAGG(+ATATAT)GTACCAGCTGCAGGCAGCAGGCCAGCGCCAG NGG 10 8 17 8 24 0 100 5 10
    240607012105_bsku TCTTACCCTCAATGCCAAACAGCTGGCACTGG(+ATATAT)GTACCTAGGTTCAGGCCAGAAGCCAGCCAGG NGG 10 8 17 8 24 0 100 5 10
    240607012103_hhz9 CTTTCCCTGGCTGGCTTCTGGCCTGAACCTAG(+ATATAT)GTACCCAGTGCCAGCTGTTTGGCATTGAGGG NGG 10 8 17 8 24 0 100 5 10
    240607012102_f3fz TAGCTCTTAGCAGTGTGCTGTGCATACAAAAG(+ATATAT)GATCCTTAAGATACACTGTTTGGTACTACAT NGG 10 8 17 8 24 0 100 5 10
    240607012100_pyqq CCTTGATGTAGTACCAAACAGTGTATCTTAAG(+ATATAT)GATCCTTTTGTATGCACAGCACACTGCTAAG NGG 10 8 17 8 24 0 100 5 10
    240607012059_374y CCATCGCAGCTGTAAGAATTGAGCTGCAGAAG(+ATATAT)GTACCCTCTGTCGGGATTGTATCTCGACATC NGG 10 8 17 8 24 0 100 5 10
    240607012057_yb1z ACAGGGATGTCGAGATACAATCCCGACAGAGG(+ATATAT)GTACCTTCTGCAGCTCAATTCTTACAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240607012056_38ob AGTCATTTTGAATTTGGTCTGTGCCTTCTCTG(+ATATAT)GATCCCTCCCTAGCCTGTGCCCCGAGCCGTC NGG 10 8 17 8 24 0 100 5 10
    240607012054_zlsi CTATGGACGGCTCGGGGCACAGGCTAGGGAGG(+ATATAT)GATCCAGAGAAGGCACAGACCAAATTCAAAA NGG 10 8 17 8 24 0 100 5 10
    240607012052_jxjk CACCCACTTGAGGTCTCCCGACATTCACCCTG(+ATATAT)GTACCACACCACTTGATCTCCAGAGCAATCT NGG 10 8 17 8 24 0 100 5 10