Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607012020_6jkt GGTCTTGCTATCTATTATAATGCTAAGTGGTG(+ATATAT)GATCCCAAGATCTGGAGTCCTCACAAAGACC NGG 10 8 17 8 24 0 100 5 10
    240607012018_k198 ACTTCAAAAGTTGGTTTTTCTCCTTCTACTGG(+ATATAT)GATCCAAGGATACAACTCAGGTTATCAGACT NGG 10 8 17 8 24 0 100 5 10
    240607012016_qj3f GAACTAGTCTGATAACCTGAGTTGTATCCTTG(+ATATAT)GATCCCAGTAGAAGGAGAAAAACCAACTTTT NGG 10 8 17 8 24 0 100 5 10
    240607012014_xh1b ATTGGAAGAGCAACATGGGCCACCCCTGCTGG(+ATATAT)GTACCCAACTTATGAGCAAGAGAATGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240607012013_5gna TGGAAGCTTCCATTCTCTTGCTCATAAGTTGG(+ATATAT)GTACCCAGCAGGGGTGGCCCATGTTGCTCTT NGG 10 8 17 8 24 0 100 5 10
    240607012011_4wd4 GCTCTGCGCACAGTTTGTTCACTAGAGGCCTG(+ATATAT)GTACCTGTCTTCTCTCAGGCCTCTGCACTCA NGG 10 8 17 8 24 0 100 5 10
    240607012010_9lvl CCGTGTGAGTGCAGAGGCCTGAGAGAAGACAG(+ATATAT)GTACCAGGCCTCTAGTGAACAAACTGTGCGC NGG 10 8 17 8 24 0 100 5 10
    240607012008_nvrw TGGATCAGGAGCCATATTAAGATGACTTTGGG(+ATATAT)GATCCCAGCAGTCCCTACGTGATCACATAGC NGG 10 8 17 8 24 0 100 5 10
    240607012006_1otv CAGGAGCTATGTGATCACGTAGGGACTGCTGG(+ATATAT)GATCCCCAAAGTCATCTTAATATGGCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240607012005_9ru5 ATGATTACTAGCACTAGAAGCCAACAGCAAAG(+ATATAT)GATCCCGCGCGCAGTTGCTCTTGTTTAATCC NGG 10 8 17 8 24 0 100 5 10