Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607012003_6ukq AACCTGGATTAAACAAGAGCAACTGCGCGCGG(+ATATAT)GATCCTTTGCTGTTGGCTTCTAGTGCTAGTA NGG 10 8 17 8 24 0 100 5 10
    240607012001_7hzm TGGTCCATTTAAAGTTTCCCAGCTATGGGGTG(+ATATAT)GTACCAGTTCTGGACAAGTGCCACTGCATCA NGG 10 8 17 8 24 0 100 5 10
    240607012000_ywl4 TACTATGATGCAGTGGCACTTGTCCAGAACTG(+ATATAT)GTACCACCCCATAGCTGGGAAACTTTAAATG NGG 10 8 17 8 24 0 100 5 10
    240607011958_xww0 GGGCGGGAGTAAGGAAATGCAGGAGTTGACCG(+ATATAT)GATCCTTGTTCCTCACCTTGTACACTCACTT NGG 10 8 17 8 24 0 100 5 10
    240607011957_gisw AGTTTAAGTGAGTGTACAAGGTGAGGAACAAG(+ATATAT)GATCCGGTCAACTCCTGCATTTCCTTACTCC NGG 10 8 17 8 24 0 100 5 10
    240607011956_wx18 CATGCTATATGTATACTTCTGTTTTCTGTTGG(+ATATAT)GTACCTTGCAATGCATTCTATGCCTCATTAA NGG 10 8 17 8 24 0 100 5 10
    240607011955_zzd1 TCCATTTAATGAGGCATAGAATGCATTGCAAG(+ATATAT)GTACCCAACAGAAAACAGAAGTATACATATA NGG 10 8 17 8 24 0 100 5 10
    240607011953_smex CAAGAAAATATCATCCTGAATGAAGTATCCTG(+ATATAT)GATCCAAAAGGACATGCATGGTGCTTACTCA NGG 10 8 17 8 24 0 100 5 10
    240607011952_9nra ATCAGTGAGTAAGCACCATGCATGTCCTTTTG(+ATATAT)GATCCAGGATACTTCATTCAGGATGATATTT NGG 10 8 17 8 24 0 100 5 10
    240607011950_sc54 GGAATGATTGACCAGTGACTATCCCAATGTGG(+ATATAT)GATCCAGGAGACTAGCATGGCTGTCCTCTGA NGG 10 8 17 8 24 0 100 5 10