Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607012051_41d3 GGGCCAGATTGCTCTGGAGATCAAGTGGTGTG(+ATATAT)GTACCAGGGTGAATGTCGGGAGACCTCAAGT NGG 10 8 17 8 24 0 100 5 10
    240607012049_mp10 TCGCCAGTTCTGTTTGGAGTCCGTGTAGGTGG(+ATATAT)GATCCTGCTGTGTGTAGCACTGGCCTGGACA NGG 10 8 17 8 24 0 100 5 10
    240607012048_0nkb GGAGATGTCCAGGCCAGTGCTACACACAGCAG(+ATATAT)GATCCCACCTACACGGACTCCAAACAGAACT NGG 10 8 17 8 24 0 100 5 10
    240607012046_c1q8 TGTAGGGGGTTGAGAGGAAGGGAATGAGCCAG(+ATATAT)GTACCCTGCATTCCAAGGGAGTTTCAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240607012044_06iu CTTTGCACACTGAAACTCCCTTGGAATGCAGG(+ATATAT)GTACCTGGCTCATTCCCTTCCTCTCAACCCC NGG 10 8 17 8 24 0 100 5 10
    240607012042_6iuo GAAGGTTTAGAGAATCCGATGGGCGCTGCCTG(+ATATAT)GATCCCGGCATAGCCCCAGACACTTGGTTCC NGG 10 8 17 8 24 0 100 5 10
    240607012040_qtvn ATAAAGGAACCAAGTGTCTGGGGCTATGCCGG(+ATATAT)GATCCAGGCAGCGCCCATCGGATTCTCTAAA NGG 10 8 17 8 24 0 100 5 10
    240607012039_cuxk GCTTTAGATAAGTCCCTGACACCAAGCTTCAG(+ATATAT)GATCCAAGTGTTAACAGGAAAGAGCTGAAAT NGG 10 8 17 8 24 0 100 5 10
    240607012038_0idi CATTTATTTCAGCTCTTTCCTGTTAACACTTG(+ATATAT)GATCCTGAAGCTTGGTGTCAGGGACTTATCT NGG 10 8 17 8 24 0 100 5 10
    240607012037_50gf ATCTCCAAGTCTTCTTCCAGAGGATGCATTTG(+ATATAT)GATCCCAGAGGAGTGTTTTCTGGAAGACAAT NGG 10 8 17 8 24 0 100 5 10