Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607011948_8zxc GCCTCTCAGAGGACAGCCATGCTAGTCTCCTG(+ATATAT)GATCCCACATTGGGATAGTCACTGGTCAATC NGG 10 8 17 8 24 0 100 5 10
    240607011947_caa6 GGAAGGAATGAATAGGATGTGGCTAGCAGGAG(+ATATAT)GATCCTGCAAGCCAAAAGAGCAGCTTAGACA NGG 10 8 17 8 24 0 100 5 10
    240607011945_tyzc AATTTTGTCTAAGCTGCTCTTTTGGCTTGCAG(+ATATAT)GATCCTCCTGCTAGCCACATCCTATTCATTC NGG 10 8 17 8 24 0 100 5 10
    240607011944_0ge4 TTGTCACTCAGAGACCAGTAACTGCTTGCATG(+ATATAT)GATCCTGCTGACTGAAGCCTCAGTTACTGTT NGG 10 8 17 8 24 0 100 5 10
    240607011943_lgyl TTATGAACAGTAACTGAGGCTTCAGTCAGCAG(+ATATAT)GATCCATGCAAGCAGTTACTGGTCTCTGAGT NGG 10 8 17 8 24 0 100 5 10
    240607011942_astz CTTGTCATCTGTTCCAAGTGAGAAGTTAGCAG(+ATATAT)GATCCATGAAGTAAATACAGCAGAGCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240607011941_7m3l TGGTGCCAGAGCTCTGCTGTATTTACTTCATG(+ATATAT)GATCCTGCTAACTTCTCACTTGGAACAGATG NGG 10 8 17 8 24 0 100 5 10
    240607011939_lzzb AGCAATCCCAGAGTCTGTGACAAGCCAGAATG(+ATATAT)GTACCAGCAGCAGGACGCAGGGTCTGAGTAC NGG 10 8 17 8 24 0 100 5 10
    240607011938_kcta ACTTCGTACTCAGACCCTGCGTCCTGCTGCTG(+ATATAT)GTACCATTCTGGCTTGTCACAGACTCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240607011936_f5kf AAGAGCCACAATGATGGAGGGAGAGAATTGGG(+ATATAT)GATCCAGCATCAAACACCCCCTCACCAGGAA NGG 10 8 17 8 24 0 100 5 10