Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607011935_elhh TTGCCTTCCTGGTGAGGGGGTGTTTGATGCTG(+ATATAT)GATCCCCAATTCTCTCCCTCCATCATTGTGG NGG 10 8 17 8 24 0 100 5 10
    240607011934_d0k0 CCAATAATGAAGTCCCAGATATCAGAAAACTG(+ATATAT)GATCCTGGCCTGAGTAGGGGGTGATACACAG NGG 10 8 17 8 24 0 100 5 10
    240607011932_hxcb CCATTCTGTGTATCACCCCCTACTCAGGCCAG(+ATATAT)GATCCAGTTTTCTGATATCTGGGACTTCATT NGG 10 8 17 8 24 0 100 5 10
    240607011931_e2ri TCACCTTATTTTTGTAGTCTCTCCCTGAACTG(+ATATAT)GTACCTCACCCATTCAGCTGTCCAGAAAACT NGG 10 8 17 8 24 0 100 5 10
    240607011930_aqy8 TCTCAAGTTTTCTGGACAGCTGAATGGGTGAG(+ATATAT)GTACCAGTTCAGGGAGAGACTACAAAAATAA NGG 10 8 17 8 24 0 100 5 10
    240607011928_eo14 AGATGGGGTCTGTGATGGATTGTTACACTGTG(+ATATAT)GATCCCTGCACTTGGCATCTGCACGAGTCTA NGG 10 8 17 8 24 0 100 5 10
    240607011927_n30h TGGCCTAGACTCGTGCAGATGCCAAGTGCAGG(+ATATAT)GATCCACAGTGTAACAATCCATCACAGACCC NGG 10 8 17 8 24 0 100 5 10
    240607011925_3zbr AATGTACAGTTGCCTAACAGACAACTTCTAGG(+ATATAT)GATCCTACAAATTCCCAGGGGATCTGTACTC NGG 10 8 17 8 24 0 100 5 10
    240607011924_1enl ACCTAGAGTACAGATCCCCTGGGAATTTGTAG(+ATATAT)GATCCCTAGAAGTTGTCTGTTAGGCAACTGT NGG 10 8 17 8 24 0 100 5 10
    240607011921_whxa GGATGGACTATCCAGAGACTACCCCACCCAGG(+ATATAT)GATCCATCCCATAATCAGCCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10