Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607011905_zj9o TTTGACCGTTTGGAGTCCCAATAGATAAACAG(+ATATAT)GATCCGTGTTAAGATACATTCCCTTTGCTAT NGG 10 8 17 8 24 0 100 5 10
    240607011903_4fzs GTGGTCCCTAGGCAAAAGGAGGGGGATTCTGG(+ATATAT)GATCCTAAAAGGCTTTATTTTTTGACTTAAA NGG 10 8 17 8 24 0 100 5 10
    240607011902_t9vk ACCCCTATGAAAGGGTAGATTGACCTCCAAAG(+ATATAT)GTACCGCTACCCACAGGCTGAGAACGACTAC NGG 10 8 17 8 24 0 100 5 10
    240607011900_htdy CTAGAGTAGTCGTTCTCAGCCTGTGGGTAGCG(+ATATAT)GTACCTTTGGAGGTCAATCTACCCTTTCATA NGG 10 8 17 8 24 0 100 5 10
    240607011859_fztx TGGGTTATGGACCAGTAAGACTTTGGGTTAGG(+ATATAT)GATCCCCTAGTCTCTTCTGCCTATTGTGTGC NGG 10 8 17 8 24 0 100 5 10
    240607011857_t4km TCAGTGCACACAATAGGCAGAAGAGACTAGGG(+ATATAT)GATCCCTAACCCAAAGTCTTACTGGTCCATA NGG 10 8 17 8 24 0 100 5 10
    240607011855_wgsc CTGAGCTGAGGGTTGGCTATGGTCTCTCATGG(+ATATAT)GTACCCTGGGGATGCCAGCTACTCATGTGCA NGG 10 8 17 8 24 0 100 5 10
    240607011852_5y1b GTGAGTGCACATGAGTAGCTGGCATCCCCAGG(+ATATAT)GTACCCATGAGAGACCATAGCCAACCCTCAG NGG 10 8 17 8 24 0 100 5 10
    240607011850_j68s GCTATATTATTCTTGCCAGAATCTTGTGGAGG(+ATATAT)GATCCCCACTCATCCCCTGACTTCTATTCTT NGG 10 8 17 8 24 0 100 5 10
    240607011849_hat3 GTACAAAGAATAGAAGTCAGGGGATGAGTGGG(+ATATAT)GATCCCTCCACAAGATTCTGGCAAGAATAAT NGG 10 8 17 8 24 0 100 5 10