Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607011804_wei4 ATTCCAAGGCCAAGTCCAGTCTATGAAACAAG(+ATATAT)GTACCATACCCAGTCTGAGCATGGGATGCTA NGG 10 8 17 8 24 0 100 5 10
    240607011803_j5kb AAAACTAGCATCCCATGCTCAGACTGGGTATG(+ATATAT)GTACCTTGTTTCATAGACTGGACTTGGCCTT NGG 10 8 17 8 24 0 100 5 10
    240607011802_w5c4 GTGCACCACGGGCCGGGCTTCATCTGTGATTG(+ATATAT)GATCCAGGGAAGAATCAGCGTGAAGTATATT NGG 10 8 17 8 24 0 100 5 10
    240607011800_vusv TGCTGAATATACTTCACGCTGATTCTTCCCTG(+ATATAT)GATCCAATCACAGATGAAGCCCGGCCCGTGG NGG 10 8 17 8 24 0 100 5 10
    240607011758_poe1 AAACATGGGGACTAATAACTTAGAGACAGTTG(+ATATAT)GTACCGTGTCTAATTTCTGCCTAAAGAGATG NGG 10 8 17 8 24 0 100 5 10
    240607011757_iu9d GAAAGCATCTCTTTAGGCAGAAATTAGACACG(+ATATAT)GTACCAACTGTCTCTAAGTTATTAGTCCCCA NGG 10 8 17 8 24 0 100 5 10
    240607011756_dh8y AAAAAATGGGCTATGAAATTATTTTACATCTG(+ATATAT)GATCCCTGTATTATAAAGTCTGTCCCCAAAC NGG 10 8 17 8 24 0 100 5 10
    240607011754_t4q3 ACGAAGTTTGGGGACAGACTTTATAATACAGG(+ATATAT)GATCCAGATGTAAAATAATTTCATAGCCCAT NGG 10 8 17 8 24 0 100 5 10
    240607011753_pk0z TCAGGTCTACATGGAACCTAGAATTTCAGCTG(+ATATAT)GATCCTGAGAGCTGGTCTTCAACAAGCGAAG NGG 10 8 17 8 24 0 100 5 10
    240607011752_ztb8 CTGACCTTCGCTTGTTGAAGACCAGCTCTCAG(+ATATAT)GATCCAGCTGAAATTCTAGGTTCCATGTAGA NGG 10 8 17 8 24 0 100 5 10