Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607011734_s0xm GGGCTCTGCTCAGCATTGTCTGAGGGGTTGGG(+ATATAT)GTACCCACACAGGTGCTTGCTGCTTCCCACG NGG 10 8 17 8 24 0 100 5 10
    240607011732_m90m AATGCCGTGGGAAGCAGCAAGCACCTGTGTGG(+ATATAT)GTACCCCAACCCCTCAGACAATGCTGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240607011730_gvya CACCAAGCCTGATGACCTGAGTTAGACCTCGG(+ATATAT)GATCCATGAGGTAGAAGAAGAGAAATGAGCC NGG 10 8 17 8 24 0 100 5 10
    240607011729_oqd3 TAAAGGGCTCATTTCTCTTCTTCTACCTCATG(+ATATAT)GATCCCGAGGTCTAACTCAGGTCATCAGGCT NGG 10 8 17 8 24 0 100 5 10
    240607011728_0ge7 GAGGTTCTGGGTTCAATTCCCATCTGTAATGG(+ATATAT)GATCCGATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240607011726_y6lj GCTGTCTTCAGACACACCAGAAGAGGGCATCG(+ATATAT)GATCCCATTACAGATGGGAATTGAACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240607011725_3hc5 GCAGGAAGTCCTGAGGTTAGGGACTGGCTATG(+ATATAT)GTACCCAGGACCCGGTCAGTAGTACCTGCTG NGG 10 8 17 8 24 0 100 5 10
    240607011723_i834 TGAGACAGCAGGTACTACTGACCGGGTCCTGG(+ATATAT)GTACCATAGCCAGTCCCTAACCTCAGGACTT NGG 10 8 17 8 24 0 100 5 10
    240607011721_nzge GAGAGTGTCTTTTCTGCCTAAGCTTGGCAATG(+ATATAT)GATCCCGGAAAGAGACGGCTCTCCCGGCTGC NGG 10 8 17 8 24 0 100 5 10
    240607011719_gpvb CATGAGCAGCCGGGAGAGCCGTCTCTTTCCGG(+ATATAT)GATCCATTGCCAAGCTTAGGCAGAAAAGACA NGG 10 8 17 8 24 0 100 5 10