Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607011701_n0rz TTTCTCTATTGGAGACAATGGAGGCTGACAGG(+ATATAT)GATCCCAGTGGGAGAGTCAGGCCTCAGGCCC NGG 10 8 17 8 24 0 100 5 10
    240607011659_vonr ACTCTGGGCCTGAGGCCTGACTCTCCCACTGG(+ATATAT)GATCCCTGTCAGCCTCCATTGTCTCCAATAG NGG 10 8 17 8 24 0 100 5 10
    240607011657_3t5l CATGAGCTCATCACCTCTTCATCCTATCCCAG(+ATATAT)GTACCACGGCCCTTAAGGCAGGGCAGCCTTG NGG 10 8 17 8 24 0 100 5 10
    240607011655_4czi CAGCTCAAGGCTGCCCTGCCTTAAGGGCCGTG(+ATATAT)GTACCTGGGATAGGATGAAGAGGTGATGAGC NGG 10 8 17 8 24 0 100 5 10
    240607011653_telr GTTCCTTTATAAAAGGAAACCGGACTGCACTG(+ATATAT)GATCCCAGGCAGCTTCCGGGCCCCAATCTGG NGG 10 8 17 8 24 0 100 5 10
    240607011651_34b8 ATGCCCCAGATTGGGGCCCGGAAGCTGCCTGG(+ATATAT)GATCCAGTGCAGTCCGGTTTCCTTTTATAAA NGG 10 8 17 8 24 0 100 5 10
    240607011649_dr81 AACTGTGCCCTGTACCATCGTGGGGGCTGGTG(+ATATAT)GTACCATGCCTGTGCCCACTCTAACCTCAAT NGG 10 8 17 8 24 0 100 5 10
    240607011648_pfob ACTCCATTGAGGTTAGAGTGGGCACAGGCATG(+ATATAT)GTACCACCAGCCCCCACGATGGTACAGGGCA NGG 10 8 17 8 24 0 100 5 10
    240607011647_kyzi TTCAGGGGACCAAGATGTTGGGCATAAATCAG(+ATATAT)GATCCTACATGGTGCTGCCCTGCTCATAACT NGG 10 8 17 8 24 0 100 5 10
    240607011646_61mq TTCCCAGTTATGAGCAGGGCAGCACCATGTAG(+ATATAT)GATCCTGATTTATGCCCAACATCTTGGTCCC NGG 10 8 17 8 24 0 100 5 10