Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607011559_e0gt CTCCCTTGTATCCTGTACAAGACGCCAGCGCG(+ATATAT)GATCCCACGCTTCGTAAGAAAGGAGTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240607011557_wiea CAAGGAAAGCACTCCTTTCTTACGAAGCGTGG(+ATATAT)GATCCGCGCTGGCGTCTTGTACAGGATACAA NGG 10 8 17 8 24 0 100 5 10
    240607011556_r0lf TCAGGTTGTCACAGGAATACCCCACTCTGCTG(+ATATAT)GTACCAATTTGTCTTAGTCAGGGTTTCTATT NGG 10 8 17 8 24 0 100 5 10
    240607011555_g5l0 GCAGGAATAGAAACCCTGACTAAGACAAATTG(+ATATAT)GTACCAGCAGAGTGGGGTATTCCTGTGACAA NGG 10 8 17 8 24 0 100 5 10
    240607011553_8osy CAAAAGTCAATCTCCAACCGTCCACTGTCTGG(+ATATAT)GATCCAACTCACGATCTTCTGGGCTCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240607011552_mnn9 GCCCTTGGAGGAGCCCAGAAGATCGTGAGTTG(+ATATAT)GATCCCAGACAGTGGACGGTTGGAGATTGAC NGG 10 8 17 8 24 0 100 5 10
    240607011551_uzf3 ATTGCAGATTATGCACAAGGACCACAGTTCAG(+ATATAT)GTACCAAATCTCAAGTGGAAGTCAGATTATT NGG 10 8 17 8 24 0 100 5 10
    240607011549_4ek7 GACACAATAATCTGACTTCCACTTGAGATTTG(+ATATAT)GTACCTGAACTGTGGTCCTTGTGCATAATCT NGG 10 8 17 8 24 0 100 5 10
    240607011548_n4a8 ATCTATAGTCCTAGGGCTAGGGAGACAGAGAG(+ATATAT)GATCCCTGGAGCATGGTGGCAGATGAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240607011546_uhx5 TCATTACTCTTCATCTGCCACCATGCTCCAGG(+ATATAT)GATCCTCTCTGTCTCCCTAGCCCTAGGACTA NGG 10 8 17 8 24 0 100 5 10