Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607011458_cxzc TGAGTCACTTTCTATATGTGATAAAGCATGGG(+ATATAT)GTACCTTCCCACTGGAGCGCAGCAAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240607011457_1pdc AACACAGAGCCTTGCTGCGCTCCAGTGGGAAG(+ATATAT)GTACCCCATGCTTTATCACATATAGAAAGTG NGG 10 8 17 8 24 0 100 5 10
    240607011455_f6lx CCTGTAAAAACCTGTCCTGTCCTATAGTTGGG(+ATATAT)GATCCACAATGCCTGGATCTCCTAGGCCCAA NGG 10 8 17 8 24 0 100 5 10
    240607011454_3r5f GCCACTTGGGCCTAGGAGATCCAGGCATTGTG(+ATATAT)GATCCCCAACTATAGGACAGGACAGGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607011452_mjef ACGGAAGGGTAATTAGGAAGGGTTAAGAGCTG(+ATATAT)GATCCACGGGCCCAGAGGGAGGTAAGAGTGT NGG 10 8 17 8 24 0 100 5 10
    240607011450_5qsv ATGATACACTCTTACCTCCCTCTGGGCCCGTG(+ATATAT)GATCCAGCTCTTAACCCTTCCTAATTACCCT NGG 10 8 17 8 24 0 100 5 10
    240607011449_kw7j AGTAGCTGAAAAAAACCAGACAGACAGAGCAG(+ATATAT)GTACCAGGACTGACTACAGCTCAGGAGTCCA NGG 10 8 17 8 24 0 100 5 10
    240607011447_xssa TAAGATGGACTCCTGAGCTGTAGTCAGTCCTG(+ATATAT)GTACCTGCTCTGTCTGTCTGGTTTTTTTCAG NGG 10 8 17 8 24 0 100 5 10
    240607011445_zji4 GGAAGTAAGTCTTGCTGAAAAGCCACACCAAG(+ATATAT)GATCCTAGCAGTAGGCGGGTAAGTGACTCCA NGG 10 8 17 8 24 0 100 5 10
    240607011444_aelh GGGGCTGGAGTCACTTACCCGCCTACTGCTAG(+ATATAT)GATCCTTGGTGTGGCTTTTCAGCAAGACTTA NGG 10 8 17 8 24 0 100 5 10