Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607011412_2qqe GAGGCTCCTGAATTGTTTATTGTGTAGACATG(+ATATAT)GATCCTGTGAAGGAAAAACTTAAACCCGAGG NGG 10 8 17 8 24 0 100 5 10
    240607011411_kyea ATTACCCTCGGGTTTAAGTTTTTCCTTCACAG(+ATATAT)GATCCATGTCTACACAATAAACAATTCAGGA NGG 10 8 17 8 24 0 100 5 10
    240607011409_0f99 TTTCTAAGTCAGGTAGCTAAGACTGTCTCCTG(+ATATAT)GTACCCGGTGTACATTCTTGGGATCCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240607011407_577v AACAAGAGAAGGATCCCAAGAATGTACACCGG(+ATATAT)GTACCAGGAGACAGTCTTAGCTACCTGACTT NGG 10 8 17 8 24 0 100 5 10
    240607011406_j5ci ACTGTCTCCTGGTACCCGGTGTACATTCTTGG(+ATATAT)GATCCTTCTCTTGTTGGCCTATCCGTTAGTT NGG 10 8 17 8 24 0 100 5 10
    240607011405_wo6k TGCTGAACTAACGGATAGGCCAACAAGAGAAG(+ATATAT)GATCCCAAGAATGTACACCGGGTACCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240607011403_jfnr GCTGAAGAGCCACCTCTCTACATAGCTCACAG(+ATATAT)GATCCTCCCCAGACACCAAGCTGAACATTCC NGG 10 8 17 8 24 0 100 5 10
    240607011401_axof TGGCTGGAATGTTCAGCTTGGTGTCTGGGGAG(+ATATAT)GATCCTGTGAGCTATGTAGAGAGGTGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240607011400_03sp CAATCCCAGCACTGGGCAGGCAGAGACGGGAG(+ATATAT)GATCCCTGGGGCTTGCTGACCTGCAAACACA NGG 10 8 17 8 24 0 100 5 10
    240607011357_tay9 CTGGCTGTGTTTGCAGGTCAGCAAGCCCCAGG(+ATATAT)GATCCTCCCGTCTCTGCCTGCCCAGTGCTGG NGG 10 8 17 8 24 0 100 5 10