Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607011449_kw7j AGTAGCTGAAAAAAACCAGACAGACAGAGCAG(+ATATAT)GTACCAGGACTGACTACAGCTCAGGAGTCCA NGG 10 8 17 8 24 0 100 5 10
    240607011447_xssa TAAGATGGACTCCTGAGCTGTAGTCAGTCCTG(+ATATAT)GTACCTGCTCTGTCTGTCTGGTTTTTTTCAG NGG 10 8 17 8 24 0 100 5 10
    240607011445_zji4 GGAAGTAAGTCTTGCTGAAAAGCCACACCAAG(+ATATAT)GATCCTAGCAGTAGGCGGGTAAGTGACTCCA NGG 10 8 17 8 24 0 100 5 10
    240607011444_aelh GGGGCTGGAGTCACTTACCCGCCTACTGCTAG(+ATATAT)GATCCTTGGTGTGGCTTTTCAGCAAGACTTA NGG 10 8 17 8 24 0 100 5 10
    240607011442_7evy TGCCCATGGTCACTGACCTGAGAGGGGTTAGG(+ATATAT)GTACCTTTCACAGAAGACAGGGCTCAGTAAA NGG 10 8 17 8 24 0 100 5 10
    240607011441_zhqe ACCACTTTACTGAGCCCTGTCTTCTGTGAAAG(+ATATAT)GTACCCTAACCCCTCTCAGGTCAGTGACCAT NGG 10 8 17 8 24 0 100 5 10
    240607011440_zye8 TTCTCGGTTAAGGTTGGACAGTCATTTCATTG(+ATATAT)GTACCAGACCACATTTGCAGCAAGATTTCAC NGG 10 8 17 8 24 0 100 5 10
    240607011438_vwly ACAAGGTGAAATCTTGCTGCAAATGTGGTCTG(+ATATAT)GTACCAATGAAATGACTGTCCAACCTTAACC NGG 10 8 17 8 24 0 100 5 10
    240607011436_z2gp AAGAGCCACACCCAACAAGACATGGGGTAGGG(+ATATAT)GATCCTGTGTCATACTTGAGGCTCTGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240607011435_0zmf GCGCCAAAGCCAGAGCCTCAAGTATGACACAG(+ATATAT)GATCCCCTACCCCATGTCTTGTTGGGTGTGG NGG 10 8 17 8 24 0 100 5 10