Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607011530_4dge TCCTCAGAAAATTGGTCATAGTACTACCGGAG(+ATATAT)GATCCAGCAATACTTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240607011528_qkbc TTCTGGATATATGCCCAGGAGAAGTATTGCTG(+ATATAT)GATCCTCCGGTAGTACTATGACCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240607011526_lshc GGATGGACCATGTAGAGACTGCCATATCCAGG(+ATATAT)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240607011525_hy9i GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+ATATAT)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240607011523_6tg9 CACAGTTTAGTTTTTTGGGGGGAGTGGGTGGG(+ATATAT)GTACCTTAGGTGTATTAACAGCAACAGATCT NGG 10 8 17 8 24 0 100 5 10
    240607011521_f7bu AAGTGAGATCTGTTGCTGTTAATACACCTAAG(+ATATAT)GTACCCCACCCACTCCCCCCAAAAAACTAAA NGG 10 8 17 8 24 0 100 5 10
    240607011520_62cx AAGTTTGAGACTGTAAGAACTAATGTGTGCTG(+ATATAT)GATCCACTGACTAACTCTCCAATTTTATATA NGG 10 8 17 8 24 0 100 5 10
    240607011518_ut4s TCTGATATATAAAATTGGAGAGTTAGTCAGTG(+ATATAT)GATCCAGCACACATTAGTTCTTACAGTCTCA NGG 10 8 17 8 24 0 100 5 10
    240607011516_o8uv TTATTTTCTACTAGAAAATTCCATTTCCTCTG(+ATATAT)GATCCAAGCTACATGAAATAAATCTAAAAGG NGG 10 8 17 8 24 0 100 5 10
    240607011515_9i53 ACATACCTTTTAGATTTATTTCATGTAGCTTG(+ATATAT)GATCCAGAGGAAATGGAATTTTCTAGTAGAA NGG 10 8 17 8 24 0 100 5 10