Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607011611_vssj TAGTTCTAACTTAAACCACTTGGTGATTGGTG(+ATATAT)GTACCGTCAGGTGCTGGGCTTCAAAAACTTG NGG 10 8 17 8 24 0 100 5 10
    240607011610_l67j CTTGCCAAGTTTTTGAAGCCCAGCACCTGACG(+ATATAT)GTACCACCAATCACCAAGTGGTTTAAGTTAG NGG 10 8 17 8 24 0 100 5 10
    240607011609_npla GTGTCAAATTCTGTGAACCAGGGCCAACACTG(+ATATAT)GTACCAGCTTCACCCTGACCTGGGGCCACTG NGG 10 8 17 8 24 0 100 5 10
    240607011608_tzqe GAGGGCAGTGGCCCCAGGTCAGGGTGAAGCTG(+ATATAT)GTACCAGTGTTGGCCCTGGTTCACAGAATTT NGG 10 8 17 8 24 0 100 5 10
    240607011606_4kwg TATAGCTAAGTTTACACCAACCAAATTATTTG(+ATATAT)GTACCAATTCTTGTAAATACTGGGTATGCTG NGG 10 8 17 8 24 0 100 5 10
    240607011605_bth3 TACCCCAGCATACCCAGTATTTACAAGAATTG(+ATATAT)GTACCAAATAATTTGGTTGGTGTAAACTTAG NGG 10 8 17 8 24 0 100 5 10
    240607011604_dljs CTCCCTTGTATCCTGTACAAGACGCCAGCGCG(+ATATAT)GATCCCACGCTTCGTAAGAAAGGAGTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240607011602_0r2j CAAGGAAAGCACTCCTTTCTTACGAAGCGTGG(+ATATAT)GATCCGCGCTGGCGTCTTGTACAGGATACAA NGG 10 8 17 8 24 0 100 5 10
    240607011601_jph1 TATAGCTAAGTTTACACCAACCAAATTATTTG(+ATATAT)GTACCAATTCTTGTAAATACTGGGTATGCTG NGG 10 8 17 8 24 0 100 5 10
    240607011600_7v6j TACCCCAGCATACCCAGTATTTACAAGAATTG(+ATATAT)GTACCAAATAATTTGGTTGGTGTAAACTTAG NGG 10 8 17 8 24 0 100 5 10