Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607011644_frqi AGACCTCATTATTTATGTGTTTGTATGTGTGG(+ATATAT)GTACCCAGAGACCAGAAGAGGAAGAGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240607011642_oly2 GGAGGTCAGACTCTTCCTCTTCTGGTCTCTGG(+ATATAT)GTACCCACACATACAAACACATAAATAATGA NGG 10 8 17 8 24 0 100 5 10
    240607011640_bmu1 GCTCACAAGCATCTGTAATTCCAGTTCCAGGG(+ATATAT)GATCCAACACCCTCTTCTGGTTGGTCTCCAT NGG 10 8 17 8 24 0 100 5 10
    240607011638_50mm TTCTCATGGAGACCAACCAGAAGAGGGTGTTG(+ATATAT)GATCCCCTGGAACTGGAATTACAGATGCTTG NGG 10 8 17 8 24 0 100 5 10
    240607011637_9w8o AAGATGCCATGCACACTGTCAGAGGACAAAGG(+ATATAT)GATCCTCAGTGTCACCCAGCTGCAGGTCGCT NGG 10 8 17 8 24 0 100 5 10
    240607011636_cwoz ACACCAGCGACCTGCAGCTGGGTGACACTGAG(+ATATAT)GATCCCTTTGTCCTCTGACAGTGTGCATGGC NGG 10 8 17 8 24 0 100 5 10
    240607011634_ovze GAACTTGCTGCTAAGCCAGACAGCCTGAGTTG(+ATATAT)GATCCCTGGGACTCATGGCCTGCTGCCGGCT NGG 10 8 17 8 24 0 100 5 10
    240607011631_8kij AAGACAGCCGGCAGCAGGCCATGAGTCCCAGG(+ATATAT)GATCCAACTCAGGCTGTCTGGCTTAGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240607011630_e50u TGGACCCCCAAAAGTTCATGCACTGACGGCTG(+ATATAT)GATCCTCGGGCCGTAGAGTTCAGTGTAGGAT NGG 10 8 17 8 24 0 100 5 10
    240607011627_ro3t CTCCCATCCTACACTGAACTCTACGGCCCGAG(+ATATAT)GATCCAGCCGTCAGTGCATGAACTTTTGGGG NGG 10 8 17 8 24 0 100 5 10