Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607011150_sjwf TTTCAGGACACCTTGTGTCATGTGTGTTCAGG(+ATATAT)GATCCTAACTTGGATCTTCATGATTGCAAAG NGG 10 8 17 8 24 0 100 5 10
    240607011149_7n8c TCTGACTTTGCAATCATGAAGATCCAAGTTAG(+ATATAT)GATCCCTGAACACACATGACACAAGGTGTCC NGG 10 8 17 8 24 0 100 5 10
    240607011148_3hb3 AACCTTCCATAGTCTGAGCTTTACAGGAGCAG(+ATATAT)GATCCCCAGCCATCTTCCCTCACTCCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240607011146_h2jn TCACACTGGAGGAGTGAGGGAAGATGGCTGGG(+ATATAT)GATCCTGCTCCTGTAAAGCTCAGACTATGGA NGG 10 8 17 8 24 0 100 5 10
    240607011145_cffg TTAGGTCAAATTGAACCTAGGCAAGGACAAAG(+ATATAT)GTACCTTCAGTGAGGAGACCAGGACGGAGGG NGG 10 8 17 8 24 0 100 5 10
    240607011144_b8qf AGTTTCCCTCCGTCCTGGTCTCCTCACTGAAG(+ATATAT)GTACCTTTGTCCTTGCCTAGGTTCAATTTGA NGG 10 8 17 8 24 0 100 5 10
    240607011141_ubfc TGAACATCATAGCCTTGCCGTTAAGTCCCCAG(+ATATAT)GTACCTCCAACCTCCCTGCTGTCATGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240607011139_pfm5 CACACATGCCCATGACAGCAGGGAGGTTGGAG(+ATATAT)GTACCTGGGGACTTAACGGCAAGGCTATGAT NGG 10 8 17 8 24 0 100 5 10
    240607011138_cc4x CGGGTGGCCAAGAACGACGGGGTGCCTCCTAG(+ATATAT)GATCCCAGGACTTACTGGGGCTTTTCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240607011136_29cr CAGCACAACTGAAAAGCCCCAGTAAGTCCTGG(+ATATAT)GATCCTAGGAGGCACCCCGTCGTTCTTGGCC NGG 10 8 17 8 24 0 100 5 10