Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607011048_kgl8 TCAGAGGTGGGACAGAGAGCTCGGCCACTCAG(+ATATAT)GATCCATCATGCAAGCAAGCAGGGTTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240607011047_tq49 CGGCCGGAAGAACCCTGCTTGCTTGCATGATG(+ATATAT)GATCCTGAGTGGCCGAGCTCTCTGTCCCACC NGG 10 8 17 8 24 0 100 5 10
    240607011046_gxr1 CCTGGAAACAACCAGGATCCTATCCATTCAAG(+ATATAT)GTACCATGGGGCAGGTCCAATCTTCAAACCA NGG 10 8 17 8 24 0 100 5 10
    240607011043_nvlp GCCATTGGTTTGAAGATTGGACCTGCCCCATG(+ATATAT)GTACCTTGAATGGATAGGATCCTGGTTGTTT NGG 10 8 17 8 24 0 100 5 10
    240607011042_6hem TTCAACTTTTAAAACAGAAGAGGCGTTGCTAG(+ATATAT)GATCCCAGACTCTCTGACATCTAAGGCCGAC NGG 10 8 17 8 24 0 100 5 10
    240607011040_5ros CCTGTGTCGGCCTTAGATGTCAGAGAGTCTGG(+ATATAT)GATCCTAGCAACGCCTCTTCTGTTTTAAAAG NGG 10 8 17 8 24 0 100 5 10
    240607011039_ccq0 GGTTATAAAGACTGCTATCACCTTTGGCAGTG(+ATATAT)GTACCAAGGAACAGTATGTATGAATGTGATG NGG 10 8 17 8 24 0 100 5 10
    240607011037_xtv5 AAGATCATCACATTCATACATACTGTTCCTTG(+ATATAT)GTACCACTGCCAAAGGTGATAGCAGTCTTTA NGG 10 8 17 8 24 0 100 5 10
    240607011036_ld2e ACAATAAGATCCCAAAGACACAGCAACATTCG(+ATATAT)GATCCACGAGTTAGAGGACTTTCATGCCTAG NGG 10 8 17 8 24 0 100 5 10
    240607011035_xxz6 AGGATCTAGGCATGAAAGTCCTCTAACTCGTG(+ATATAT)GATCCGAATGTTGCTGTGTCTTTGGGATCTT NGG 10 8 17 8 24 0 100 5 10