Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607011005_nkwr GTAGTAGTAGTAGTAGTTCCATGTAGGGGCAG(+ATATAT)GATCCTTTGGAAAAGTACCGTATGTTCCTAG NGG 10 8 17 8 24 0 100 5 10
    240607011003_z82n GGGGTCTAGGAACATACGGTACTTTTCCAAAG(+ATATAT)GATCCTGCCCCTACATGGAACTACTACTACT NGG 10 8 17 8 24 0 100 5 10
    240607011002_dluf TTGTTCCTGCATGAGATGGCTTTGGGTGTCTG(+ATATAT)GTACCACTCCTTCCCTTCCCCCTACCTGGCC NGG 10 8 17 8 24 0 100 5 10
    240607011001_crsb CAATCGGCCAGGTAGGGGGAAGGGAAGGAGTG(+ATATAT)GTACCAGACACCCAAAGCCATCTCATGCAGG NGG 10 8 17 8 24 0 100 5 10
    240607010959_85wl GTAGTAGTAGTAGTAGTTCCATGTAGGGGCAG(+ATATAT)GATCCTTTGGAAAAGTACCGTATGTTCCTAG NGG 10 8 17 8 24 0 100 5 10
    240607010957_czu5 GGGGTCTAGGAACATACGGTACTTTTCCAAAG(+ATATAT)GATCCTGCCCCTACATGGAACTACTACTACT NGG 10 8 17 8 24 0 100 5 10
    240607010955_alnw GGGGTCAAGTTGGTCACAGGTAGGAATCTGGG(+ATATAT)GTACCATCAGGGTCTAGTGAATGAAGGATTC NGG 10 8 17 8 24 0 100 5 10
    240607010954_mx2r AGGCTGAATCCTTCATTCACTAGACCCTGATG(+ATATAT)GTACCCCAGATTCCTACCTGTGACCAACTTG NGG 10 8 17 8 24 0 100 5 10
    240607010953_clg6 GTTTGAAAGGTGAACTGTAAGACTCTTCACTG(+ATATAT)GATCCTTAATGTTTCAGTGGTCCAGTCTTCA NGG 10 8 17 8 24 0 100 5 10
    240607010952_i6xn TTTGTTGAAGACTGGACCACTGAAACATTAAG(+ATATAT)GATCCAGTGAAGAGTCTTACAGTTCACCTTT NGG 10 8 17 8 24 0 100 5 10