Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010933_5dv3 AAAAAGCTGCTTCTAGTGTGTGAGGTTTCCGG(+ATATAT)GTACCTTTTAGTTCCTCATAAAGTTGCAGAC NGG 10 8 17 8 24 0 100 5 10
    240607010932_3rdd TCAGAGTCTGCAACTTTATGAGGAACTAAAAG(+ATATAT)GTACCCGGAAACCTCACACACTAGAAGCAGC NGG 10 8 17 8 24 0 100 5 10
    240607010931_3wee AAAAGGTTTGTTTTCTTTCATAATTGCATCTG(+ATATAT)GTACCCAGCAGGAAGTACCTGGCCCCTCCTT NGG 10 8 17 8 24 0 100 5 10
    240607010929_59hh CGAACAAGGAGGGGCCAGGTACTTCCTGCTGG(+ATATAT)GTACCAGATGCAATTATGAAAGAAAACAAAC NGG 10 8 17 8 24 0 100 5 10
    240607010926_s8ih TGTTATGAAATATAATCTGGTGGCCGGCCGGG(+ATATAT)GTACCTGGAGGACCCATGCCAAGCTGTGCAA NGG 10 8 17 8 24 0 100 5 10
    240607010925_misu CTCAGTTGCACAGCTTGGCATGGGTCCTCCAG(+ATATAT)GTACCCCGGCCGGCCACCAGATTATATTTCA NGG 10 8 17 8 24 0 100 5 10
    240607010923_kbxu CCTTTGACCATCTCTGTTTTGGGTTCAGTTTG(+ATATAT)GATCCTGAAACTTTCCTTCCCAGAAGGTTTG NGG 10 8 17 8 24 0 100 5 10
    240607010922_hd5s GGGCACAAACCTTCTGGGAAGGAAAGTTTCAG(+ATATAT)GATCCAAACTGAACCCAAAACAGAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240607010921_4eio AAATCACTGACTGTATCCCTGGGGTTGTTCTG(+ATATAT)GATCCTCTGCATCAGGTCACCTCATGGGTGA NGG 10 8 17 8 24 0 100 5 10
    240607010920_supk CTCCCTCACCCATGAGGTGACCTGATGCAGAG(+ATATAT)GATCCAGAACAACCCCAGGGATACAGTCAGT NGG 10 8 17 8 24 0 100 5 10