Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010901_b5o4 TCCACCACTTGGAAAGCGGCGGTGACCCAGAG(+ATATAT)GATCCTGGCTGGCAGCCCTTTACTGCTCAAG NGG 10 8 17 8 24 0 100 5 10
    240607010859_xkyn AAAGCCTTGAGCAGTAAAGGGCTGCCAGCCAG(+ATATAT)GATCCTCTGGGTCACCGCCGCTTTCCAAGTG NGG 10 8 17 8 24 0 100 5 10
    240607010858_nhd4 AGGGCATCCTGCCGCGCCGAGGGAGCATCTGG(+ATATAT)GATCCACACGTCTCCTGTCCCTGCAGACCTG NGG 10 8 17 8 24 0 100 5 10
    240607010856_c0vj GTGCTCAGGTCTGCAGGGACAGGAGACGTGTG(+ATATAT)GATCCCAGATGCTCCCTCGGCGCGGCAGGAT NGG 10 8 17 8 24 0 100 5 10
    240607010855_2wq1 GCACCTGCCTAGGGGAGGCAGCTTTCTGAGGG(+ATATAT)GATCCAGAGACAATGGCCAGTGGCAAATGTT NGG 10 8 17 8 24 0 100 5 10
    240607010854_k8ju TCACAAACATTTGCCACTGGCCATTGTCTCTG(+ATATAT)GATCCCCTCAGAAAGCTGCCTCCCCTAGGCA NGG 10 8 17 8 24 0 100 5 10
    240607010852_g6tz AGAACACAGCCTGTGCCTGAATTAATGGCTGG(+ATATAT)GATCCATGGATGGAACACTTGGGTATGCACA NGG 10 8 17 8 24 0 100 5 10
    240607010850_hu5a GGCTTTGTGCATACCCAAGTGTTCCATCCATG(+ATATAT)GATCCCAGCCATTAATTCAGGCACAGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240607010848_4tb4 TGGGTTTGTGGCCACTCCAGGGGTTTACCCTG(+ATATAT)GATCCTCTGCCTCCGAGGGGCTTGTCCTAAT NGG 10 8 17 8 24 0 100 5 10
    240607010846_ix9u GAAAGATTAGGACAAGCCCCTCGGAGGCAGAG(+ATATAT)GATCCAGGGTAAACCCCTGGAGTGGCCACAA NGG 10 8 17 8 24 0 100 5 10