Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010813_3g8h ATGTTCTTCAGGAAAAAAGAATGTTGAACAAG(+ATATAT)GTACCAGAAAAGGAACTAGAACACATACTGC NGG 10 8 17 8 24 0 100 5 10
    240607010811_ir15 GGAGTGCAGTATGTGTTCTAGTTCCTTTTCTG(+ATATAT)GTACCTTGTTCAACATTCTTTTTTCCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240607010810_dle5 TCAAAAAGGAGGAGGAAGGCGTCTTGTATGTG(+ATATAT)GATCCACAGCAACAGGCGTTCAGTTAACATC NGG 10 8 17 8 24 0 100 5 10
    240607010809_5awv GCATGGATGTTAACTGAACGCCTGTTGCTGTG(+ATATAT)GATCCACATACAAGACGCCTTCCTCCTCCTT NGG 10 8 17 8 24 0 100 5 10
    240607010807_9vv9 GGAAACTTCCTACGTGTTCGTAGCTACACTGG(+ATATAT)GTACCAAGGTGCGCGCCGCCGGCGGTCCAAC NGG 10 8 17 8 24 0 100 5 10
    240607010806_kvl3 ACGAAGTTGGACCGCCGGCGGCGCGCACCTTG(+ATATAT)GTACCCAGTGTAGCTACGAACACGTAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240607010805_uie3 TAGTAAGCATAAGGGGCTGCCTTCATCTTATG(+ATATAT)GTACCTTGGACACATCATCCTATGGTCACAG NGG 10 8 17 8 24 0 100 5 10
    240607010803_r2d0 CTGTCCTGTGACCATAGGATGATGTGTCCAAG(+ATATAT)GTACCATAAGATGAAGGCAGCCCCTTATGCT NGG 10 8 17 8 24 0 100 5 10
    240607010801_w9ol GAGTGTGATCACCAACCACATCCGCTCCTAAG(+ATATAT)GATCCACATCTTATCCTGCAGGTAACAGACA NGG 10 8 17 8 24 0 100 5 10
    240607010800_gjbm CACCGTGTCTGTTACCTGCAGGATAAGATGTG(+ATATAT)GATCCTTAGGAGCGGATGTGGTTGGTGATCA NGG 10 8 17 8 24 0 100 5 10