Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010726_x7l8 GCTCTCTGGGTGGCTTACGCTGGATTGTAGTG(+ATATAT)GATCCAGCCCCACTTGGGGACCTGCCATGTA NGG 10 8 17 8 24 0 100 5 10
    240607010725_g18h AGAGCTACATGGCAGGTCCCCAAGTGGGGCTG(+ATATAT)GATCCACTACAATCCAGCGTAAGCCACCCAG NGG 10 8 17 8 24 0 100 5 10
    240607010723_m1aq CTTTTAAACATGGGAGACACTTGGGTATCATG(+ATATAT)GATCCTAGACCAGGCTCTTTGTAAATAAATG NGG 10 8 17 8 24 0 100 5 10
    240607010722_vkuj TGTTACATTTATTTACAAAGAGCCTGGTCTAG(+ATATAT)GATCCATGATACCCAAGTGTCTCCCATGTTT NGG 10 8 17 8 24 0 100 5 10
    240607010720_nre4 CCTTCGAAAACAAAAACTGAAACAAAAGGCTG(+ATATAT)GATCCTGTGTCCTGGGACCTCTTTGCACTCA NGG 10 8 17 8 24 0 100 5 10
    240607010719_f72g GAATGTGAGTGCAAAGAGGTCCCAGGACACAG(+ATATAT)GATCCAGCCTTTTGTTTCAGTTTTTGTTTTC NGG 10 8 17 8 24 0 100 5 10
    240607010718_hj8z TGCTGGCCAAATTCTCCACTATTGAAGGAATG(+ATATAT)GTACCGATGAAAATGCAAGACACATTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240607010717_zt5l CAAGTCAACAAATGTGTCTTGCATTTTCATCG(+ATATAT)GTACCATTCCTTCAATAGTGGAGAATTTGGC NGG 10 8 17 8 24 0 100 5 10
    240607010714_ul9i CCAGCAGCCTGAAAAAATTGAAATGCCCAAGG(+ATATAT)GATCCACCTTGACTTTTGTTTGTGAGAACAT NGG 10 8 17 8 24 0 100 5 10
    240607010713_kszy TCCTGATGTTCTCACAAACAAAAGTCAAGGTG(+ATATAT)GATCCCTTGGGCATTTCAATTTTTTCAGGCT NGG 10 8 17 8 24 0 100 5 10