Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010836_r0js GGGCTGAGCTCCACCCTGACTCTGAGGATAGG(+ATATAT)GTACCCTCGGGGAGGTAGTGTTGCTCTTTTG NGG 10 8 17 8 24 0 100 5 10
    240607010835_6xh4 CAGCAGGACATGGTGTAGAAAGAACTGGTTTG(+ATATAT)GTACCTGGAGTAGAAAGAGGAAGCTCAGGGC NGG 10 8 17 8 24 0 100 5 10
    240607010833_8qdn CTGCAGCCCTGAGCTTCCTCTTTCTACTCCAG(+ATATAT)GTACCAAACCAGTTCTTTCTACACCATGTCC NGG 10 8 17 8 24 0 100 5 10
    240607010831_a9vg CAGTGTATGTAAATCCAAGATGGATCTTGGAG(+ATATAT)GATCCTGAAGAACTTTGTCATAGATATAGGC NGG 10 8 17 8 24 0 100 5 10
    240607010830_0de2 GCTCTGCCTATATCTATGACAAAGTTCTTCAG(+ATATAT)GATCCTCCAAGATCCATCTTGGATTTACATA NGG 10 8 17 8 24 0 100 5 10
    240607010828_8m4i TGCAAAATTAATAAAATCATTGTTTTTGAGGG(+ATATAT)GTACCAAATCAGCGTCTGCGTTTTGAACTAC NGG 10 8 17 8 24 0 100 5 10
    240607010827_2j87 AATCTGTAGTTCAAAACGCAGACGCTGATTTG(+ATATAT)GTACCCCTCAAAAACAATGATTTTATTAATT NGG 10 8 17 8 24 0 100 5 10
    240607010826_yz5w CCAAGGTCTGGAAACTTCTTAATATGAATGTG(+ATATAT)GATCCGGAAGCAGCCATTTCCAAAAGTCTTC NGG 10 8 17 8 24 0 100 5 10
    240607010824_ziuh TTGCAGAAGACTTTTGGAAATGGCTGCTTCCG(+ATATAT)GATCCACATTCATATTAAGAAGTTTCCAGAC NGG 10 8 17 8 24 0 100 5 10
    240607010823_uxw4 CGTCATTTTCACATTAGGAGTTGGCATTTTGG(+ATATAT)GTACCTCCCAAAATCATACAGATGAGAAAAC NGG 10 8 17 8 24 0 100 5 10