Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010628_cq4p GATGTGTCTAGCTAGCTAGCCAGAATGCTCAG(+ATATAT)GATCCTCTATGACCTTATTAGCTTTAACTGT NGG 10 8 17 8 24 0 100 5 10
    240607010627_1fmk AGTTGACAGTTAAAGCTAATAAGGTCATAGAG(+ATATAT)GATCCTGAGCATTCTGGCTAGCTAGCTAGAC NGG 10 8 17 8 24 0 100 5 10
    240607010626_x798 AGTGGCAGGGGTCAGCTTTCTCCATCATGTAG(+ATATAT)GTACCAGATATCAAACCCAGGTTTTCAGTTT NGG 10 8 17 8 24 0 100 5 10
    240607010625_lp7p TACCCAAACTGAAAACCTGGGTTTGATATCTG(+ATATAT)GTACCTACATGATGGAGAAAGCTGACCCCTG NGG 10 8 17 8 24 0 100 5 10
    240607010623_43tz TGTGGGAATCAGTTCTCTTCTTCCAGCATGTG(+ATATAT)GATCCTGGGACTTGAACTTAGGTCTTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240607010621_juvm CCATAGCCTGAAGACCTAAGTTCAAGTCCCAG(+ATATAT)GATCCACATGCTGGAAGAAGAGAACTGATTC NGG 10 8 17 8 24 0 100 5 10
    240607010619_zcqm AAGTATGCCAGCAGCAGACTCCTGAAGGTCAG(+ATATAT)GATCCCTCACCTGAACTCCAAAACTATCAAA NGG 10 8 17 8 24 0 100 5 10
    240607010618_d36j AGGTCTTTGATAGTTTTGGAGTTCAGGTGAGG(+ATATAT)GATCCTGACCTTCAGGAGTCTGCTGCTGGCA NGG 10 8 17 8 24 0 100 5 10
    240607010616_5cvy CCTTGCTTACTGCGATCTATGAATAAACTAGG(+ATATAT)GATCCTCCAAGGCAAACTGGATTGTTGCAAT NGG 10 8 17 8 24 0 100 5 10
    240607010614_k4am ATGTCATTGCAACAATCCAGTTTGCCTTGGAG(+ATATAT)GATCCCTAGTTTATTCATAGATCGCAGTAAG NGG 10 8 17 8 24 0 100 5 10