Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010525_8b9g TAAACAGCATAAGCGGATAGATTCTACTTGGG(+ATATAT)GATCCTGATGTCAAAGTAAGGACACACGGGA NGG 10 8 17 8 24 0 100 5 10
    240607010524_8ale CACCCTCCCGTGTGTCCTTACTTTGACATCAG(+ATATAT)GATCCCCAAGTAGAATCTATCCGCTTATGCT NGG 10 8 17 8 24 0 100 5 10
    240607010523_m2i6 ATGACCTGCTGATCCATGGGCTGACAACTTCG(+ATATAT)GTACCAGGTTCACACTCTTTGGCCATGAAGT NGG 10 8 17 8 24 0 100 5 10
    240607010520_r24s GCGGGACTTCATGGCCAAAGAGTGTGAACCTG(+ATATAT)GTACCGAAGTTGTCAGCCCATGGATCAGCAG NGG 10 8 17 8 24 0 100 5 10
    240607010519_d6b6 AAAGGAAAAAAAAAGTGTGGGTGTGATGTCTG(+ATATAT)GATCCGTGTTTGCCTGCAGTAGCCTGTGAGA NGG 10 8 17 8 24 0 100 5 10
    240607010518_t2bi GGATGTCTCACAGGCTACTGCAGGCAAACACG(+ATATAT)GATCCAGACATCACACCCACACTTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607010516_y0c1 GGTTTGAGAAGCCCAATCTTGTCGGAGACGGG(+ATATAT)GATCCTCATTGATGGGAGGACTGAGGCCTGC NGG 10 8 17 8 24 0 100 5 10
    240607010515_6xuv CCACTGCAGGCCTCAGTCCTCCCATCAATGAG(+ATATAT)GATCCCCGTCTCCGACAAGATTGGGCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240607010514_bgs9 GTCCTCTACCCCCTGTGATGTTGAGCGTGCTG(+ATATAT)GATCCTGGGGGCTGTCGGAGGCTGGTTCAGC NGG 10 8 17 8 24 0 100 5 10
    240607010510_gtyy CTGGCGCTGAACCAGCCTCCGACAGCCCCCAG(+ATATAT)GATCCAGCACGCTCAACATCACAGGGGGTAG NGG 10 8 17 8 24 0 100 5 10