Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010435_452n ATTTCCAAGAAGGAAAGGTCGGCCTTTGTGCG(+ATATAT)GTACCACAGCCTCAGTTTCCTAGTCTGCAGG NGG 10 8 17 8 24 0 100 5 10
    240607010434_girt TTCACCCTGCAGACTAGGAAACTGAGGCTGTG(+ATATAT)GTACCGCACAAAGGCCGACCTTTCCTTCTTG NGG 10 8 17 8 24 0 100 5 10
    240607010432_8sg5 TCTAAAAGATGAAATATCCACTATCCAAAGGG(+ATATAT)GATCCTCAGAGGGCAACATAGTCAGCGACCA NGG 10 8 17 8 24 0 100 5 10
    240607010431_6s9v AGGACTTTGACTAGCTGGAGAGGTCTGGGGAG(+ATATAT)GATCCAGGGCAGCACAAATGGGATGAAGCTG NGG 10 8 17 8 24 0 100 5 10
    240607010428_md9q CTTTGCAGCTTCATCCCATTTGTGCTGCCCTG(+ATATAT)GATCCTCCCCAGACCTCTCCAGCTAGTCAAA NGG 10 8 17 8 24 0 100 5 10
    240607010427_ncpm TCACTGGCCAGTTAGCCTAAGCAAAGTAAATG(+ATATAT)GTACCTAAAAGAACACATAAGGTTCCCCTCT NGG 10 8 17 8 24 0 100 5 10
    240607010426_v6gb GGGCCAGAGGGGAACCTTATGTGTTCTTTTAG(+ATATAT)GTACCATTTACTTTGCTTAGGCTAACTGGCC NGG 10 8 17 8 24 0 100 5 10
    240607010425_yvv2 GCTGGTACAGACTCCTATGGAGTTTTGCTGAG(+ATATAT)GTACCAACCATGGGAGAGCACAAGATGTTTG NGG 10 8 17 8 24 0 100 5 10
    240607010423_ps31 CTCTCCAAACATCTTGTGCTCTCCCATGGTTG(+ATATAT)GTACCTCAGCAAAACTCCATAGGAGTCTGTA NGG 10 8 17 8 24 0 100 5 10
    240607010422_c2m0 GAGGATGCTCGGAGTGGCACCAGAGATGGCAG(+ATATAT)GTACCACCCTGACTCTAGTTGTCTGTTGCTG NGG 10 8 17 8 24 0 100 5 10