Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010338_j3qt GTCACATATTGGCTTGGGCTGCCTTACTCTTG(+ATATAT)GTACCTAAATCTCTGGGGGTGCTAGGATGGG NGG 10 8 17 8 24 0 100 5 10
    240607010336_8tai TGGCCTAGACCTTTATATGCTGATCAGGTTGG(+ATATAT)GTACCAACTGTCAGCCTGTCTGCTGTCTCCC NGG 10 8 17 8 24 0 100 5 10
    240607010335_qpi5 GAGATGGGAGACAGCAGACAGGCTGACAGTTG(+ATATAT)GTACCCAACCTGATCAGCATATAAAGGTCTA NGG 10 8 17 8 24 0 100 5 10
    240607010334_hnvs CAGATGACAGCTTACACCAATCAGGCTGATTG(+ATATAT)GTACCAAAAAGCACACATTTCTGCTTTTATT NGG 10 8 17 8 24 0 100 5 10
    240607010333_9b9w AATAAAATAAAAGCAGAAATGTGTGCTTTTTG(+ATATAT)GTACCAATCAGCCTGATTGGTGTAAGCTGTC NGG 10 8 17 8 24 0 100 5 10
    240607010330_lmuy GAGCCTCCGCGGAGCCGTCCGTCTGTCCCTGG(+ATATAT)GATCCTCGAGCGTCTGGCGGTTCCCTCAGCA NGG 10 8 17 8 24 0 100 5 10
    240607010329_az0z TGTGGTGCTGAGGGAACCGCCAGACGCTCGAG(+ATATAT)GATCCCAGGGACAGACGGACGGCTCCGCGGA NGG 10 8 17 8 24 0 100 5 10
    240607010328_odxg TCATGACCCATCTTCAGAGACTGACATTTCAG(+ATATAT)GATCCATATTAAAGCATTCATTTTCTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240607010327_q7y4 AATGTAACAAAGAAAATGAATGCTTTAATATG(+ATATAT)GATCCTGAAATGTCAGTCTCTGAAGATGGGT NGG 10 8 17 8 24 0 100 5 10
    240607010325_pe7a TATAATCCTAAATCAGTATCTGTATGGAATGG(+ATATAT)GTACCTTGCCCTGCAGTTTCCATGAGGAATT NGG 10 8 17 8 24 0 100 5 10