Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010254_3dwa ATGTTCAAAATGTGCCTCAAGTTTATTTTAGG(+ATATAT)GATCCGATTATTAGATCTGTGAAAGTTAAGG NGG 10 8 17 8 24 0 100 5 10
    240607010253_httl CAAACTGCTGCCACTCTACAAGCAAATGCCTG(+ATATAT)GATCCCTTCTGATGTGCAGTAACCAGGACTC NGG 10 8 17 8 24 0 100 5 10
    240607010251_krhe AAGGTGAGTCCTGGTTACTGCACATCAGAAGG(+ATATAT)GATCCAGGCATTTGCTTGTAGAGTGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240607010249_bao9 TGTGCCTCAAAGTTCGGTTGGGGCTACCTTGG(+ATATAT)GATCCCAAACAGCTAATATGGTCACCAACTG NGG 10 8 17 8 24 0 100 5 10
    240607010247_kicr ATAGCCAGTTGGTGACCATATTAGCTGTTTGG(+ATATAT)GATCCCAAGGTAGCCCCAACCGAACTTTGAG NGG 10 8 17 8 24 0 100 5 10
    240607010245_bl0k CTCTGGAGACATCTTCAGCCTGAGGAGGCTGG(+ATATAT)GTACCAAGGAGGAAGAACCCAGCTGGCTTTA NGG 10 8 17 8 24 0 100 5 10
    240607010244_qc9h GGTGGTAAAGCCAGCTGGGTTCTTCCTCCTTG(+ATATAT)GTACCCAGCCTCCTCAGGCTGAAGATGTCTC NGG 10 8 17 8 24 0 100 5 10
    240607010242_vmg6 ATCCCTCAGGCTCTGCTGTGGACTAGTTCCAG(+ATATAT)GATCCCTGTCACCAAGTATATGAAAACCTGA NGG 10 8 17 8 24 0 100 5 10
    240607010241_cxcf CATCCTCAGGTTTTCATATACTTGGTGACAGG(+ATATAT)GATCCTGGAACTAGTCCACAGCAGAGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240607010240_85w6 GTTTAAAAAAAGAAAAAAAGAAAAAAAAACAG(+ATATAT)GTACCTGAAATCCCAGCACTTGAGAGGTTGA NGG 10 8 17 8 24 0 100 5 10