Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010509_1pk1 AACGGCCTCAACAGGGCCGAGAGGCTGACAGG(+ATATAT)GTACCTGGAGTCCAGTTACTCACTGGCTGTC NGG 10 8 17 8 24 0 100 5 10
    240607010507_wpdd ACTCAGACAGCCAGTGAGTAACTGGACTCCAG(+ATATAT)GTACCCTGTCAGCCTCTCGGCCCTGTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240607010505_4d3v TCTACCCCATGTGGTCTGCCTCTGGTGTGGTG(+ATATAT)GTACCTGGTCCTTTTTATACCCCTTCCACCT NGG 10 8 17 8 24 0 100 5 10
    240607010504_4rou AGCCAAGGTGGAAGGGGTATAAAAAGGACCAG(+ATATAT)GTACCACCACACCAGAGGCAGACCACATGGG NGG 10 8 17 8 24 0 100 5 10
    240607010502_clcw CCTTCTGCACTCAGCCTTTCCTTGGGGCTAGG(+ATATAT)GATCCATCACATGGGGTAAGGCCTAGCCTAA NGG 10 8 17 8 24 0 100 5 10
    240607010501_a9cl TTGGATTAGGCTAGGCCTTACCCCATGTGATG(+ATATAT)GATCCCTAGCCCCAAGGAAAGGCTGAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240607010459_zd41 GGTGGGATGAGTACAGGGCCTTCAGGTCTCAG(+ATATAT)GATCCCAAAGCCTCTGTTGCCCCATGGGGAT NGG 10 8 17 8 24 0 100 5 10
    240607010458_or0m TAGTCATCCCCATGGGGCAACAGAGGCTTTGG(+ATATAT)GATCCTGAGACCTGAAGGCCCTGTACTCATC NGG 10 8 17 8 24 0 100 5 10
    240607010456_wnm2 TTTTCTAAATATCATCAGTCCACATTTGGTTG(+ATATAT)GATCCAGACATCCAGAGCTGGGGGTGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240607010455_5zs2 CAGCCCTCTGCACCCCCAGCTCTGGATGTCTG(+ATATAT)GATCCAACCAAATGTGGACTGATGATATTTA NGG 10 8 17 8 24 0 100 5 10