Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010613_ix3f CCTTGCTTACTGCGATCTATGAATAAACTAGG(+ATATAT)GATCCTCCAAGGCAAACTGGATTGTTGCAAT NGG 10 8 17 8 24 0 100 5 10
    240607010611_laz0 ATGTCATTGCAACAATCCAGTTTGCCTTGGAG(+ATATAT)GATCCCTAGTTTATTCATAGATCGCAGTAAG NGG 10 8 17 8 24 0 100 5 10
    240607010610_znfo AGTGACAGGGTAGAATTCAGGCTCTTACATAG(+ATATAT)GATCCAAGTTTCATCCATGGACAGGGAGTTC NGG 10 8 17 8 24 0 100 5 10
    240607010609_p4ry CCTCTGAACTCCCTGTCCATGGATGAAACTTG(+ATATAT)GATCCTATGTAAGAGCCTGAATTCTACCCTG NGG 10 8 17 8 24 0 100 5 10
    240607010607_bidg TGTTTTAAAAATCAAATTCGGCTGAGCTGTTG(+ATATAT)GATCCGGGTGACACAGGTGACAAGCAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240607010605_0cnc AGGGGACTCTTGCTTGTCACCTGTGTCACCCG(+ATATAT)GATCCAACAGCTCAGCCGAATTTGATTTTTA NGG 10 8 17 8 24 0 100 5 10
    240607010604_ufzg CTGAGCAGGCAAGCAGTCATCCCAAGAAGATG(+ATATAT)GTACCCACTGTCCAGTTGACAGTCAATTTGG NGG 10 8 17 8 24 0 100 5 10
    240607010602_xkrl CAAAACCAAATTGACTGTCAACTGGACAGTGG(+ATATAT)GTACCATCTTCTTGGGATGACTGCTTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240607010601_lgzp TTCTTATTTCAGTATTCCCAGAGCTAAGAATG(+ATATAT)GTACCTAATATACAGTAAGCCCTCAATAAAT NGG 10 8 17 8 24 0 100 5 10
    240607010600_3mwz TGAGTATTTATTGAGGGCTTACTGTATATTAG(+ATATAT)GTACCATTCTTAGCTCTGGGAATACTGAAAT NGG 10 8 17 8 24 0 100 5 10