Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010558_a2do TGAACCTAAGGCACAATAGGAAAGGGAGGTGG(+ATATAT)GTACCAGTGTGGGTCACAGCCTGAAGCCTGT NGG 10 8 17 8 24 0 100 5 10
    240607010557_ox79 GGGGTACAGGCTTCAGGCTGTGACCCACACTG(+ATATAT)GTACCCACCTCCCTTTCCTATTGTGCCTTAG NGG 10 8 17 8 24 0 100 5 10
    240607010555_0527 GGGGATCCAGCATCCTCCTCTGAGCTACTTTG(+ATATAT)GTACCACATACAGAAGTGTGCATATACATGC NGG 10 8 17 8 24 0 100 5 10
    240607010554_ukh4 TTCTTGCATGTATATGCACACTTCTGTATGTG(+ATATAT)GTACCAAAGTAGCTCAGAGGAGGATGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240607010552_816h CATCACAAACATGGGAAACTCTGGTCCCAGGG(+ATATAT)GATCCAGCATCCTCCTCTGAGCTACTTTGGT NGG 10 8 17 8 24 0 100 5 10
    240607010551_e0ak GTGGTACCAAAGTAGCTCAGAGGAGGATGCTG(+ATATAT)GATCCCCTGGGACCAGAGTTTCCCATGTTTG NGG 10 8 17 8 24 0 100 5 10
    240607010550_vh8l AGAGGCAGACACAGGGAGCAGCTCCAGGAACG(+ATATAT)GTACCGTGGAGGATTCTGGCCAGGGCATCCC NGG 10 8 17 8 24 0 100 5 10
    240607010548_vi2p ACCCTGGGATGCCCTGGCCAGAATCCTCCACG(+ATATAT)GTACCGTTCCTGGAGCTGCTCCCTGTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240607010547_yze6 ATAAATAAAAACTCAAGGAAAATAAATACATG(+ATATAT)GATCCTATGCGGTTGGAGGGCGCGGGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240607010546_24ng CTACATCCAGCCCGCGCCCTCCAACCGCATAG(+ATATAT)GATCCATGTATTTATTTTCCTTGAGTTTTTA NGG 10 8 17 8 24 0 100 5 10