Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010658_wcvw CAAACATACAGTCTACTCATTCCATAAAAAAG(+ATATAT)GTACCTTTCCTTCCTTTCCTTTTACTCATCC NGG 10 8 17 8 24 0 100 5 10
    240607010657_ir8u GAAAGGGATGAGTAAAAGGAAAGGAAGGAAAG(+ATATAT)GTACCTTTTTTATGGAATGAGTAGACTGTAT NGG 10 8 17 8 24 0 100 5 10
    240607010656_1go1 ACTGAATGGCAGCTGTGGACAGGATCTATGTG(+ATATAT)GTACCCTAAATGAATAAGTGAGATACTACCC NGG 10 8 17 8 24 0 100 5 10
    240607010654_qdpu ACAGGGGGTAGTATCTCACTTATTCATTTAGG(+ATATAT)GTACCACATAGATCCTGTCCACAGCTGCCAT NGG 10 8 17 8 24 0 100 5 10
    240607010653_n09k ATGGATGGAAGAAGACGGTGTCAAGGAATGAG(+ATATAT)GTACCTGACGCTTCTCGGCATCTCTGCGATC NGG 10 8 17 8 24 0 100 5 10
    240607010652_f2fi ATGCAGATCGCAGAGATGCCGAGAAGCGTCAG(+ATATAT)GTACCTCATTCCTTGACACCGTCTTCTTCCA NGG 10 8 17 8 24 0 100 5 10
    240607010650_2ykj GTCACCGAGTGTTGGTACTTTATAACTGCATG(+ATATAT)GTACCCTAGAAATGATCGTTTCATCTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240607010649_zu0p CATTGAAAGAAGATGAAACGATCATTTCTAGG(+ATATAT)GTACCATGCAGTTATAAAGTACCAACACTCG NGG 10 8 17 8 24 0 100 5 10
    240607010647_1afm ATAATTCATGGGGGGGGGGGGGCGCGCTAGGG(+ATATAT)GTACCATGATGGATGTGTGGAGGTCAATGGA NGG 10 8 17 8 24 0 100 5 10
    240607010646_repu AGTCATCCATTGACCTCCACACATCCATCATG(+ATATAT)GTACCCCTAGCGCGCCCCCCCCCCCCCATGA NGG 10 8 17 8 24 0 100 5 10