Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010741_t24o AGCTGGGCCGGGGCCCAAGGTGCCCGAGAGAG(+ATATAT)GTACCCTCGGGAGGACTGGGGTGGGGGTGGT NGG 10 8 17 8 24 0 100 5 10
    240607010739_bdwg GGAGCACCACCCCCACCCCAGTCCTCCCGAGG(+ATATAT)GTACCTCTCTCGGGCACCTTGGGCCCCGGCC NGG 10 8 17 8 24 0 100 5 10
    240607010737_18hf GGGCCCAGTCCCAGTGCCCCAGGACTCCTGTG(+ATATAT)GATCCAACCAGCTTCCTCTCCCCCGGGACTA NGG 10 8 17 8 24 0 100 5 10
    240607010736_fkd2 AACTTTAGTCCCGGGGGAGAGGAAGCTGGTTG(+ATATAT)GATCCACAGGAGTCCTGGGGCACTGGGACTG NGG 10 8 17 8 24 0 100 5 10
    240607010734_z66t AAACCCCTGGTGCTCTCCCAGACCAGAAAAGG(+ATATAT)GTACCAGATCTCCTGGAACTGGAATTACAAG NGG 10 8 17 8 24 0 100 5 10
    240607010733_2siu AACCACTTGTAATTCCAGTTCCAGGAGATCTG(+ATATAT)GTACCCTTTTCTGGTCTGGGAGAGCACCAGG NGG 10 8 17 8 24 0 100 5 10
    240607010732_986v AGTGTGCAGACAAACGGGACATGCGATCTGAG(+ATATAT)GATCCGAAGCCATACTGCTAACTAGACCCTG NGG 10 8 17 8 24 0 100 5 10
    240607010731_paz3 CCCTGCAGGGTCTAGTTAGCAGTATGGCTTCG(+ATATAT)GATCCTCAGATCGCATGTCCCGTTTGTCTGC NGG 10 8 17 8 24 0 100 5 10
    240607010729_2ubw TCCGGCTCCTGGGTGCCACTACCAGTCCTGTG(+ATATAT)GTACCCTGTCTATGCCTGGGACCTTCTGCTG NGG 10 8 17 8 24 0 100 5 10
    240607010727_lfgg GCACCCAGCAGAAGGTCCCAGGCATAGACAGG(+ATATAT)GTACCACAGGACTGGTAGTGGCACCCAGGAG NGG 10 8 17 8 24 0 100 5 10