Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010757_9k5s AAAAACCCATCAGCCTGAGGCTTTTGCCTTGG(+ATATAT)GTACCTGCCCATTTTATAGTTAAATAACACT NGG 10 8 17 8 24 0 100 5 10
    240607010756_f1u5 ACATTAGTGTTATTTAACTATAAAATGGGCAG(+ATATAT)GTACCCAAGGCAAAAGCCTCAGGCTGATGGG NGG 10 8 17 8 24 0 100 5 10
    240607010755_7jil TGCTTCTGCCAAATGACTAATATGGCCTGCTG(+ATATAT)GATCCCAAGCACACGAGGGCTTTGAGGTCAC NGG 10 8 17 8 24 0 100 5 10
    240607010753_gulz TGAGTGTGACCTCAAAGCCCTCGTGTGCTTGG(+ATATAT)GATCCAGCAGGCCATATTAGTCATTTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240607010751_9bln CTAGGCCAGAGAGAAGGTGGGCCTGAGTCCAG(+ATATAT)GATCCAACCCTCCTAGGTGGCATCACCAGGG NGG 10 8 17 8 24 0 100 5 10
    240607010750_yqmq CGCCTCCCTGGTGATGCCACCTAGGAGGGTTG(+ATATAT)GATCCTGGACTCAGGCCCACCTTCTCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240607010748_gz4q ATAATGGCCCATCTTTTGGGATACGGTCCTTG(+ATATAT)GATCCACTCTTATTTCATCCCCAGTCCTTGT NGG 10 8 17 8 24 0 100 5 10
    240607010747_q21m CCTGCACAAGGACTGGGGATGAAATAAGAGTG(+ATATAT)GATCCAAGGACCGTATCCCAAAAGATGGGCC NGG 10 8 17 8 24 0 100 5 10
    240607010745_wjar CAGCCACTCAGCCCTTCCCTCCTCCAGCCCTG(+ATATAT)GTACCCCCGCGTTCCAAAGCCGCCCAGTTCT NGG 10 8 17 8 24 0 100 5 10
    240607010742_9sec CTCCCAGAACTGGGCGGCTTTGGAACGCGGGG(+ATATAT)GTACCAGGGCTGGAGGAGGGAAGGGCTGAGT NGG 10 8 17 8 24 0 100 5 10