Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607010454_ur1o GGTGGGATGAGTACAGGGCCTTCAGGTCTCAG(+ATATAT)GATCCCAAAGCCTCTGTTGCCCCATGGGGAT NGG 10 8 17 8 24 0 100 5 10
    240607010452_s4hh TAGTCATCCCCATGGGGCAACAGAGGCTTTGG(+ATATAT)GATCCTGAGACCTGAAGGCCCTGTACTCATC NGG 10 8 17 8 24 0 100 5 10
    240607010451_a5cf GGGGCATGCAGCTGTGTGCTCGCAGCCCTGTG(+ATATAT)GTACCTTGAGGGTGGGCAGGAGGACGGGACC NGG 10 8 17 8 24 0 100 5 10
    240607010450_z24a AGTCAGGTCCCGTCCTCCTGCCCACCCTCAAG(+ATATAT)GTACCACAGGGCTGCGAGCACACAGCTGCAT NGG 10 8 17 8 24 0 100 5 10
    240607010448_wgki CTGTGAAAGGTGCCAGCTGCCAGCTGAGAAGG(+ATATAT)GTACCCAGGGGCTGTGGCACACCAGGACTGT NGG 10 8 17 8 24 0 100 5 10
    240607010445_02lu GGGGAACAGTCCTGGTGTGCCACAGCCCCTGG(+ATATAT)GTACCCTTCTCAGCTGGCAGCTGGCACCTTT NGG 10 8 17 8 24 0 100 5 10
    240607010443_tgw0 CAGGGTCTGGTCCAAGCTGGCCTGGCATTCTG(+ATATAT)GATCCTCCTGCCCCAGCCTCCCCAGTGCCAT NGG 10 8 17 8 24 0 100 5 10
    240607010442_c29w TGGTAATGGCACTGGGGAGGCTGGGGCAGGAG(+ATATAT)GATCCAGAATGCCAGGCCAGCTTGGACCAGA NGG 10 8 17 8 24 0 100 5 10
    240607010439_vdh9 CAGAGCAAACATGATGGCCTGCCATCTCCTGG(+ATATAT)GATCCCCCAAGGGTGACTTCCACTCCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240607010437_w0ty GAAAACCAGAGGAGTGGAAGTCACCCTTGGGG(+ATATAT)GATCCCAGGAGATGGCAGGCCATCATGTTTG NGG 10 8 17 8 24 0 100 5 10