Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607011019_mgxt AGCTCACAACTGACTATAACTCCAGTTCTGGG(+ATATAT)GATCCTACACCATCACACAGACCTATGTGTT NGG 10 8 17 8 24 0 100 5 10
    240607011017_nb3h TTGCCAACACATAGGTCTGTGTGATGGTGTAG(+ATATAT)GATCCCCAGAACTGGAGTTATAGTCAGTTGT NGG 10 8 17 8 24 0 100 5 10
    240607011016_bkl3 GTGAAGATGGATACCTAGTCCTTCCCCGCTTG(+ATATAT)GTACCCACCAAGAGTAACAGGGAGATATGTG NGG 10 8 17 8 24 0 100 5 10
    240607011014_qsfm CCGCACACATATCTCCCTGTTACTCTTGGTGG(+ATATAT)GTACCAAGCGGGGAAGGACTAGGTATCCATC NGG 10 8 17 8 24 0 100 5 10
    240607011013_hcsa CTTGGGGGCCTATCCCTTAGAACTCTGGACTG(+ATATAT)GATCCTGAGCCATTTTTCGAAATGAAAAGTG NGG 10 8 17 8 24 0 100 5 10
    240607011012_vl6b CTTCCCACTTTTCATTTCGAAAAATGGCTCAG(+ATATAT)GATCCAGTCCAGAGTTCTAAGGGATAGGCCC NGG 10 8 17 8 24 0 100 5 10
    240607011011_5gjd GGTGGGTGGGCAGGAGCTGGAGCCATTCTTAG(+ATATAT)GTACCAACCCTCCCAGACTCCTAGCTAGACA NGG 10 8 17 8 24 0 100 5 10
    240607011009_7mpl TGCTGTGTCTAGCTAGGAGTCTGGGAGGGTTG(+ATATAT)GTACCTAAGAATGGCTCCAGCTCCTGCCCAC NGG 10 8 17 8 24 0 100 5 10
    240607011008_heu5 TTGTTCCTGCATGAGATGGCTTTGGGTGTCTG(+ATATAT)GTACCACTCCTTCCCTTCCCCCTACCTGGCC NGG 10 8 17 8 24 0 100 5 10
    240607011007_dx1f CAATCGGCCAGGTAGGGGGAAGGGAAGGAGTG(+ATATAT)GTACCAGACACCCAAAGCCATCTCATGCAGG NGG 10 8 17 8 24 0 100 5 10