Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607005636_zo8d TTCCTACTTCCAAACACCAGCTGGCACCAAAG(+ATATAT)GATCCCTTCAACTTTGCAGGACAGATCCCGG NGG 10 8 17 8 24 0 100 5 10
    240607005633_qaor CCTCTCATGGCGCTGCTACCGATGACTCCCAG(+ATATAT)GATCCCAGACGTTCAGAACCAGATTCTCATT NGG 10 8 17 8 24 0 100 5 10
    240607005632_gszb AAAGCAATGAGAATCTGGTTCTGAACGTCTGG(+ATATAT)GATCCTGGGAGTCATCGGTAGCAGCGCCATG NGG 10 8 17 8 24 0 100 5 10
    240607005629_xzhu CCTGGCAGGCAGGTGGGCGGTCTGCCTCCAGG(+ATATAT)GATCCTGGGACCCTGATGGCGATCGTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240607005627_wf4i GCATGACAGGACGATCGCCATCAGGGTCCCAG(+ATATAT)GATCCCTGGAGGCAGACCGCCCACCTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240607005626_nbjz TACTCATCTTGTACCAAATTGTCTGATGGCAG(+ATATAT)GATCCAGAAGGTATTTTGTCAGCCCAGCTCC NGG 10 8 17 8 24 0 100 5 10
    240607005624_nf4h TATATGGAGCTGGGCTGACAAAATACCTTCTG(+ATATAT)GATCCTGCCATCAGACAATTTGGTACAAGAT NGG 10 8 17 8 24 0 100 5 10
    240607005623_x679 TCTGAAGGATGCCCTGGGATAGATGCAGAATG(+ATATAT)GTACCTAGGAACCTGCTGACATTTTGCAGAA NGG 10 8 17 8 24 0 100 5 10
    240607005621_a1jq CACCCTTCTGCAAAATGTCAGCAGGTTCCTAG(+ATATAT)GTACCATTCTGCATCTATCCCAGGGCATCCT NGG 10 8 17 8 24 0 100 5 10
    240607005619_9b4x GAACCCAGAGCGTGGTGCTCAACAGTCACCTG(+ATATAT)GTACCAGCTGACTGGATGACTCAGAAGTTAG NGG 10 8 17 8 24 0 100 5 10