Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607005655_flle TTGCCGGTGAGTAGCCACAGATGGCCATCTTG(+ATATAT)GTACCACCTCAGCCTGTGCAGGAGTCGGGAC NGG 10 8 17 8 24 0 100 5 10
    240607005654_n6yu AGGGGACGGCCAGACAGTACAGTCACCCATAG(+ATATAT)GATCCCCAGAGACATAAAGGGTATCAGGACA NGG 10 8 17 8 24 0 100 5 10
    240607005651_kn9i CTTTCTGTCCTGATACCCTTTATGTCTCTGGG(+ATATAT)GATCCTATGGGTGACTGTACTGTCTGGCCGT NGG 10 8 17 8 24 0 100 5 10
    240607005650_tbyw CACCACCGGGATCTGTCCTGCAAAGTTGAAGG(+ATATAT)GATCCTTTGGTGCCAGCTGGTGTTTGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240607005648_98fc TTCCTACTTCCAAACACCAGCTGGCACCAAAG(+ATATAT)GATCCCTTCAACTTTGCAGGACAGATCCCGG NGG 10 8 17 8 24 0 100 5 10
    240607005646_v5hu CCTCTCATGGCGCTGCTACCGATGACTCCCAG(+ATATAT)GATCCCAGACGTTCAGAACCAGATTCTCATT NGG 10 8 17 8 24 0 100 5 10
    240607005644_tfsj AAAGCAATGAGAATCTGGTTCTGAACGTCTGG(+ATATAT)GATCCTGGGAGTCATCGGTAGCAGCGCCATG NGG 10 8 17 8 24 0 100 5 10
    240607005642_fv12 CCTGGCAGGCAGGTGGGCGGTCTGCCTCCAGG(+ATATAT)GATCCTGGGACCCTGATGGCGATCGTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240607005639_lo2d GCATGACAGGACGATCGCCATCAGGGTCCCAG(+ATATAT)GATCCCTGGAGGCAGACCGCCCACCTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240607005638_eejv CACCACCGGGATCTGTCCTGCAAAGTTGAAGG(+ATATAT)GATCCTTTGGTGCCAGCTGGTGTTTGGAAGT NGG 10 8 17 8 24 0 100 5 10