Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607005803_9htb CAACACCCGAGGACGACAGGCTGTGGGGCAAG(+ATATAT)GATCCTTCGGGGGTTCCAGTCCCGAGACGGT NGG 10 8 17 8 24 0 100 5 10
    240607005801_as4n GTGCACTCATGCACACAGGCATAGAGGGCCTG(+ATATAT)GATCCTACCTGCTCATCTGGTGTCAAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240607005800_d0my TGTTCGAAGCTTGACACCAGATGAGCAGGTAG(+ATATAT)GATCCAGGCCCTCTATGCCTGTGTGCATGAG NGG 10 8 17 8 24 0 100 5 10
    240607005757_id4a GTCATGCCCCCTGACTTGTGGGATGCCCCACG(+ATATAT)GATCCACTGAAGAGCTGAGGCGGTGAACAGG NGG 10 8 17 8 24 0 100 5 10
    240607005756_7t36 AACATCCTGTTCACCGCCTCAGCTCTTCAGTG(+ATATAT)GATCCGTGGGGCATCCCACAAGTCAGGGGGC NGG 10 8 17 8 24 0 100 5 10
    240607005755_k710 ATGAAGGGTGGGACAGGTGCAGGCACACACAG(+ATATAT)GTACCCAAGAACAGAGCTCTCAGCGGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240607005753_vrhr AGTGCCCCAGCCGCTGAGAGCTCTGTTCTTGG(+ATATAT)GTACCTGTGTGTGCCTGCACCTGTCCCACCC NGG 10 8 17 8 24 0 100 5 10
    240607005752_ugtl TGATAAAGCCAAAAAAAAAAAAAGCAATATAG(+ATATAT)GTACCAGTGAACTCTGGGACAAATTCATTCC NGG 10 8 17 8 24 0 100 5 10
    240607005750_nt6w AATTTGGAATGAATTTGTCCCAGAGTTCACTG(+ATATAT)GTACCTATATTGCTTTTTTTTTTTTTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240607005749_akb4 TGTTTTTCTGTTTGTGTTCACTCCTTTTCTAG(+ATATAT)GATCCAGGCAAAGGTGTAAGCCGAGCTTTAA NGG 10 8 17 8 24 0 100 5 10