Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607005836_ox92 GCAACCTGACCACATCGCTGGTTGCCACATGG(+ATATAT)GTACCCAGCGAGGCAGCCTTGCGGCTGGGGC NGG 10 8 17 8 24 0 100 5 10
    240607005834_qf3d CTGGCTTCTGAAACAAGCAAACCCCTTTAAAG(+ATATAT)GTACCCTGGGAAGTCAAGTTTGCTAGCAGTC NGG 10 8 17 8 24 0 100 5 10
    240607005832_jm3y TCTCAGACTGCTAGCAAACTTGACTTCCCAGG(+ATATAT)GTACCTTTAAAGGGGTTTGCTTGTTTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240607005830_5de7 TGAATTACTTCATAAAACTAGATCTTTTTTTG(+ATATAT)GTACCTGTAACAAAAGCAGATATTTTATTAA NGG 10 8 17 8 24 0 100 5 10
    240607005829_y0g2 TTTATTTAATAAAATATCTGCTTTTGTTACAG(+ATATAT)GTACCAAAAAAAGATCTAGTTTTATGAAGTA NGG 10 8 17 8 24 0 100 5 10
    240607005828_sy1z AATGCCTTAGGGGCATCATACGTTCTATACGG(+ATATAT)GTACCAAAAAGCTTACACAGCTTTGTTATAG NGG 10 8 17 8 24 0 100 5 10
    240607005826_rylb TTTATCTATAACAAAGCTGTGTAAGCTTTTTG(+ATATAT)GTACCCGTATAGAACGTATGATGCCCCTAAG NGG 10 8 17 8 24 0 100 5 10
    240607005824_0vjz AGTTATACCACTGAAGAATATGAAATTCCAGG(+ATATAT)GATCCAAGAGATGAAACTCCCCTTCCCCAGT NGG 10 8 17 8 24 0 100 5 10
    240607005823_vdwv TGGCTACTGGGGAAGGGGAGTTTCATCTCTTG(+ATATAT)GATCCCTGGAATTTCATATTCTTCAGTGGTA NGG 10 8 17 8 24 0 100 5 10
    240607005822_uzcu CTGGGTTCTTACCTTTTGGGTCTTGTATTCTG(+ATATAT)GATCCGGGTTAAAGATCTCAAGTTGATTGAT NGG 10 8 17 8 24 0 100 5 10