Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607005820_8yr9 TGTAAATCAATCAACTTGAGATCTTTAACCCG(+ATATAT)GATCCAGAATACAAGACCCAAAAGGTAAGAA NGG 10 8 17 8 24 0 100 5 10
    240607005818_7dhs GATGTTGCTGAAGGACACAACCACACATTCTG(+ATATAT)GTACCCTTTTCTCTTCATTCTTGTTGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240607005817_1gup TTAAACCTCCCAACAAGAATGAAGAGAAAAGG(+ATATAT)GTACCAGAATGTGTGGTTGTGTCCTTCAGCA NGG 10 8 17 8 24 0 100 5 10
    240607005815_feym CATCTGTCTTTCTACTCCTTCAGTTGTATGGG(+ATATAT)GATCCAGGGATCTGTTAAATATTAGGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240607005812_x9p6 TCCATACTTCCCTAATATTTAACAGATCCCTG(+ATATAT)GATCCCCATACAACTGAAGGAGTAGAAAGAC NGG 10 8 17 8 24 0 100 5 10
    240607005811_rtcs GCTCCACTCCATGTGAGAGCTTTCCTTGGCAG(+ATATAT)GTACCCCATGGCTCTGGCAGCCCCAAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240607005809_q315 CCCCAAGAGCTTGGGGCTGCCAGAGCCATGGG(+ATATAT)GTACCTGCCAAGGAAAGCTCTCACATGGAGT NGG 10 8 17 8 24 0 100 5 10
    240607005808_kcq9 ATCTGGAGAAATAGTTCAGAGTTCTACATCTG(+ATATAT)GATCCACAGGCAGCAGGAAGAGAGAGCCATT NGG 10 8 17 8 24 0 100 5 10
    240607005806_zfac GACCCAATGGCTCTCTCTTCCTGCTGCCTGTG(+ATATAT)GATCCAGATGTAGAACTCTGAACTATTTCTC NGG 10 8 17 8 24 0 100 5 10
    240607005804_1yxt AGCAGACCGTCTCGGGACTGGAACCCCCGAAG(+ATATAT)GATCCTTGCCCCACAGCCTGTCGTCCTCGGG NGG 10 8 17 8 24 0 100 5 10