Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607005908_489p AATCATGGACCCAAAAGTGAGCTTCAGAAAGG(+ATATAT)GTACCCAGTTAACGGGCTCCTTCGGATGTCT NGG 10 8 17 8 24 0 100 5 10
    240607005907_7ber GGCCACTGGGCAGAAAGGCTTTATGCCACTTG(+ATATAT)GTACCTTGGTAACACACTTCAAAAGAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240607005905_vc2o TGGGGTTGCTTCTTTTGAAGTGTGTTACCAAG(+ATATAT)GTACCAAGTGGCATAAAGCCTTTCTGCCCAG NGG 10 8 17 8 24 0 100 5 10
    240607005903_cq14 GTGTGAACAATCTTCTGTTCCCCATGCGTGGG(+ATATAT)GATCCATGTCCTTAAAGAAAAATCCGCTGTC NGG 10 8 17 8 24 0 100 5 10
    240607005902_dnby AGTAAGACAGCGGATTTTTCTTTAAGGACATG(+ATATAT)GATCCCCACGCATGGGGAACAGAAGATTGTT NGG 10 8 17 8 24 0 100 5 10
    240607005900_4qlv TAGTAGCATATCTGGTTCCCAAAGAAAACCAG(+ATATAT)GTACCTGGACAGTGGACCCGCTATGGCCAGT NGG 10 8 17 8 24 0 100 5 10
    240607005858_k4k3 ATCACACTGGCCATAGCGGGTCCACTGTCCAG(+ATATAT)GTACCTGGTTTTCTTTGGGAACCAGATATGC NGG 10 8 17 8 24 0 100 5 10
    240607005857_zhms TTTCAATGGAATCTAAGGAACATCAAGTGCAG(+ATATAT)GTACCAGGAGTAGGCTCTGGGTCACAATGGG NGG 10 8 17 8 24 0 100 5 10
    240607005855_20zv CACACCCCATTGTGACCCAGAGCCTACTCCTG(+ATATAT)GTACCTGCACTTGATGTTCCTTAGATTCCAT NGG 10 8 17 8 24 0 100 5 10
    240607005854_qnqx GGTGTCAGCGTTTGCATGCTGATTATGGGGTG(+ATATAT)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10