Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607005955_dhot CAACTCAATTCTGCCACCTGCTGGTTGCTTTG(+ATATAT)GTACCTTGGTCTCTTATTCAAACCCACACCA NGG 10 8 17 8 24 0 100 5 10
    240607005954_x231 TGGGGCCTTCACAGGGGCCTGAGCACCTTTGG(+ATATAT)GATCCTGGGCTGGAACTGAAGCCTGGGTCTT NGG 10 8 17 8 24 0 100 5 10
    240607005951_8vwj CAGCTAAGACCCAGGCTTCAGTTCCAGCCCAG(+ATATAT)GATCCCAAAGGTGCTCAGGCCCCTGTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240607005949_7ca2 CACACACACACACACACACACACACACTCATG(+ATATAT)GATCCTGAGGTTGAGAGTGCTCTTCTGCCGC NGG 10 8 17 8 24 0 100 5 10
    240607005948_ua44 TTTTTGCGGCAGAAGAGCACTCTCAACCTCAG(+ATATAT)GATCCATGAGTGTGTGTGTGTGTGTGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240607005946_m4lk TATTTACAAACGAGCACACCTCTCCATTTAAG(+ATATAT)GATCCAGTTTGCAAGTGTACAGGAGGTTGTC NGG 10 8 17 8 24 0 100 5 10
    240607005945_8kxj TGTGGGACAACCTCCTGTACACTTGCAAACTG(+ATATAT)GATCCTTAAATGGAGAGGTGTGCTCGTTTGT NGG 10 8 17 8 24 0 100 5 10
    240607005944_gmdj ATTGAGGATCATTTCTAGGACAAATAAATGAG(+ATATAT)GTACCCGCTGTAGCACAGCCCGAACCCCTTG NGG 10 8 17 8 24 0 100 5 10
    240607005942_wr2j ACAGTCAAGGGGTTCGGGCTGTGCTACAGCGG(+ATATAT)GTACCTCATTTATTTGTCCTAGAAATGATCC NGG 10 8 17 8 24 0 100 5 10
    240607005941_w5ct TAGTACTTATAGTGTATAAAGAATGAGAGCTG(+ATATAT)GATCCACACATGGTTCTGGAAACTGGGAGGT NGG 10 8 17 8 24 0 100 5 10